Chaabane Amel, Chioukh Fatma Z, Chadli Zohra, Ben Fredj Nadia, Ben Ameur Karim, Ben Hmida Hayet, Boughattas Naceur A, Monastiri Kamel, Aouam Karim
Laboratory of pharmacology, Pharmacology department, faculty of medicine, university of Monastir, Avicenne Street, 5019 Monastir, Tunisia.
Intensive care and neonatal medicine department-university hospital of Monastir, 5019 Monastir, Tunisia.
Therapie. 2017 Dec;72(6):685-689. doi: 10.1016/j.therap.2017.06.004. Epub 2017 Jul 29.
Evaluate whether saliva could be a useful alternative to serum for routine therapeutic drug monitoring of caffeine in preterm infants using the enzyme multiplied immunoassay technique (EMIT) assay.
We conducted a prospective study including preterm infants (less than 34 weeks' amenorrhea) admitted to the intensive care and neonatal medicine department. All infants received 5, 10, 15, 20 and 25mg/kg/day of citrate caffeine intravenously from the first to the fifth day of birth, respectively. For each patient, two concomitant blood and saliva samples corresponding to the trough concentrations were collected 24hours after each caffeine dose. The caffeine concentrations were determined using the EMIT2000 caffeine assay.
Thirteen preterm infants were included. The saliva and the serum caffeine concentration increased proportionally to the administered dose. Saliva and serum kinetics were comparable and the saliva caffeine concentrations were correlated to the serum ones (r=0.76).
Saliva caffeine monitoring by EMIT is a valid, useful and safe alternative to serum in preterm infants.
评估在早产儿中使用酶放大免疫分析技术（EMIT）测定法时，唾液是否可作为血清的有用替代物用于咖啡因的常规治疗药物监测。
我们开展了一项前瞻性研究，纳入入住重症监护和新生儿医学科的早产儿（闭经少于34周）。所有婴儿在出生的第1天至第5天分别静脉注射5、10、15、20和25mg/kg/天的枸橼酸咖啡因。对于每位患者，在每次咖啡因剂量给药24小时后采集两份对应谷浓度的血液和唾液样本。使用EMIT2000咖啡因测定法测定咖啡因浓度。
纳入了13名早产儿。唾液和血清中的咖啡因浓度与给药剂量成比例增加。唾液和血清动力学具有可比性，唾液咖啡因浓度与血清咖啡因浓度相关（r = 0.76）。
在早产儿中，通过EMIT进行唾液咖啡因监测是一种有效、有用且安全的血清替代方法。
