Imai Takeharu, Oue Naohide, Yamamoto Yuji, Asai Ryuichi, Uraoka Naohiro, Sentani Kazuhiro, Yoshida Kazuhiro, Yasui Wataru
Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima 734-8551, Japan; Department of Surgical Oncology, Graduate School of Medicine, Gifu University, Gifu 501-1194, Japan.
Department of Molecular Pathology, Hiroshima University Institute of Biomedical and Health Sciences, Hiroshima 734-8551, Japan.
Pathol Res Pract. 2017 Nov;213(11):1388-1393. doi: 10.1016/j.prp.2017.09.009. Epub 2017 Sep 12.
Esophageal squamous cell carcinoma (ESCC) is one of the most common human cancers. We previously reported that KIFC1 is involved in gastric cancer pathogenesis and that KIFC1 plays an important role in gastric cancer spheroid colony formation. However, the significance of KIFC1 in ESCC has not been examined. In the present study, we analyzed the expression and distribution of KIFC1 in 132 ESCC cases by immunohistochemistry. In contrast to weak or no staining of KIFC1 in non-neoplastic mucosa, ESCC tissue showed stronger, more extensive KIFC1 staining. In total, 95 (72%) of 132 ESCC cases were positive for KIFC1. Immunostaining of ALDH1 was also performed, and KIFC1-positive ESCC cases were significantly frequently found in ALDH1-positive ESCC cases compared with ALDH1-negative ESCC cases. Spheroid colony formation is an effective method to characterize CSCs, thus we analyzed sphere number and size at 15days in ESCC cells downregulated for KIFC1 by siRNA transfection. Both the number and size of sphere from TE-1 cells were significantly reduced in KIFC1 siRNA-transfected TE-1 cells than in negative control siRNA-transfected cells. These results suggest that KIFC1 plays an important role in ESCC pathogenesis.
食管鳞状细胞癌（ESCC）是最常见的人类癌症之一。我们之前报道过KIFC1参与胃癌发病机制，且KIFC1在胃癌球样集落形成中起重要作用。然而，KIFC1在ESCC中的意义尚未得到研究。在本研究中，我们通过免疫组织化学分析了132例ESCC病例中KIFC1的表达和分布。与非肿瘤黏膜中KIFC1染色较弱或无染色不同，ESCC组织显示出更强、更广泛的KIFC1染色。在132例ESCC病例中，共有95例（72%）KIFC1呈阳性。我们还进行了ALDH1免疫染色，与ALDH1阴性的ESCC病例相比，在ALDH1阳性的ESCC病例中显著更频繁地发现KIFC1阳性的ESCC病例。球样集落形成是表征癌症干细胞（CSCs）特征的有效方法，因此我们通过siRNA转染分析了KIFC1下调的ESCC细胞在15天时的球状体数量和大小。与阴性对照siRNA转染的细胞相比，KIFC1 siRNA转染的TE-1细胞中TE-1细胞形成的球状体数量和大小均显著减少。这些结果表明KIFC1在ESCC发病机制中起重要作用。
