Lin Lijuan, Sun Jie, Tan Yan, Li Zhenling, Kong Fanyong, Shen Yue, Liu Chao, Chen Litian
Institute of Molecular Medicine, Medical College of Eastern Liaoning University, Dandong 118000, China.
Department of Pathology, Yanbian University, Yanji 133002, China.
Hum Pathol. 2017 Nov;69:31-37. doi: 10.1016/j.humpath.2017.09.002. Epub 2017 Sep 28.
To explore the role of NQO1 overexpression for prognostic implication in hepatocellular carcinoma (HCC), NQO1 mRNA levels were detected in HCC fresh tissue samples of HCC and nontumor tissues, respectively. One hundred fifty-six cases of HCC meeting strict follow-up criteria were selected for immunohistochemical staining of NQO1 protein. Correlations between NQO1 overexpression and clinicopathological features of HCC were evaluated using χ tests, survival rates were calculated using the Kaplan-Meier method, and the relationship between prognostic factors and patient 5-year survival was analyzed using Cox proportional hazards analysis. In results, the levels of NQO1 mRNA were significantly up-regulated in 14 fresh tissue samples of HCC. Immunohistochemical analysis showed that the NQO1 expression and overexpression rates were significantly higher in HCC samples compared with either adjacent nontumor tissues or normal liver tissues. NQO1 overexpression correlated to tumor size, venous infiltration and late pTNM stage of HCC. NQO1 overexpression was also related to low disease-free survival and 5-year survival rates. In the late-stage group, disease-free and 5-year survival rates of patients with NQO1 overexpression were significantly lower than those of patients without NQO1 expression. Further analysis using a Cox proportional hazards regression model revealed that NQO1 expression emerged as a significant independent hazard factor for the 5-year survival rate of patients with HCC. Therefore, NQO1 plays an important role in the progression of HCC. NQO1 may potentially be used as an independent biomarker for prognostic evaluation of HCC.
为探讨NQO1过表达在肝细胞癌（HCC）预后中的作用，分别检测了HCC新鲜组织样本和非肿瘤组织中NQO1 mRNA水平。选取156例符合严格随访标准的HCC患者进行NQO1蛋白免疫组化染色。采用χ检验评估NQO1过表达与HCC临床病理特征的相关性，采用Kaplan-Meier法计算生存率，采用Cox比例风险分析评估预后因素与患者5年生存率的关系。结果显示，14例HCC新鲜组织样本中NQO1 mRNA水平显著上调。免疫组化分析表明，与相邻非肿瘤组织或正常肝组织相比，HCC样本中NQO1的表达及过表达率显著更高。NQO1过表达与HCC的肿瘤大小、静脉浸润及晚期pTNM分期相关。NQO1过表达还与低无病生存率和5年生存率相关。在晚期组中，NQO1过表达患者的无病生存率和5年生存率显著低于无NQO1表达的患者。使用Cox比例风险回归模型进一步分析显示，NQO1表达是HCC患者5年生存率的一个显著独立危险因素。因此，NQO1在HCC进展中起重要作用。NQO1可能潜在地用作HCC预后评估的独立生物标志物。
