Pan Liu-Hong, Yao Min, Cai Yin, Gu Juan-Juan, Yang Xu-Li, Wang Li, Yao Deng-Fu
Liu-Hong Pan, Deng-Fu Yao, Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China.
World J Gastroenterol. 2016 Apr 14;22(14):3829-36. doi: 10.3748/wjg.v22.i14.3829.
To investigate member 3a of Wingless-type MMTV integration site family (Wnt3a) expression in cancerous and surrounding tissues and the relationship between clinicopathologic features of hepatocellular carcinoma (HCC) and Wnt3a expression.
Wnt3a expression and cellular distribution and clinicopathologic characteristics in cancerous tissue and matched surrounding tissues were analyzed in 80 HCC patients from January 2006 to August 2008 by tissue microarrays and immunohistochemistry. The overall and disease-free survival rates were estimated using the Kaplan-Meier method and compared with the log-rank test. The prognostic analysis was carried out with univariate and multivariate Cox regressions models.
The incidence of oncogenic Wnt3a expression in the cancerous group was up to 96.25% (77 of 80), which was significantly higher (χ(2) = 48.818, P < 0.001) than that in the surrounding group (46.25%, 37 of 80). Brown Wnt3a staining gradually increased with clinical staging that showed very strong staining in advanced HCC. The clinicopathologic features of high Wnt3a expression in HCC were related to poorly-differentiated grade (χ(2) = 20.211, P < 0.001), liver cirrhosis (χ(2) = 8.467, P < 0.004), hepatitis B virus (HBV) infection (χ(2) = 12.957, P < 0.001), higher tumor-node-metastasis stage (χ(2) = 22.960, P < 0.001), and 5-year survival rate (χ(2) = 15.469, P < 0.001).
Oncogenic Wnt3a expression associated with HBV infection and cirrhotic liver might be an independent prognostic factor for HCC.
研究无翅型MMTV整合位点家族成员3a（Wnt3a）在肝癌组织及其癌旁组织中的表达情况，以及肝细胞癌（HCC）的临床病理特征与Wnt3a表达之间的关系。
采用组织芯片和免疫组化方法，分析2006年1月至2008年8月期间80例HCC患者癌组织及其配对癌旁组织中Wnt3a的表达、细胞分布及临床病理特征。采用Kaplan-Meier法估计总生存率和无病生存率，并通过对数秩检验进行比较。采用单因素和多因素Cox回归模型进行预后分析。
癌组织中致癌性Wnt3a表达发生率高达96.25%（80例中的77例），显著高于癌旁组织（46.25%，80例中的37例）（χ(2)=48.818，P<0.001）。Wnt3a棕色染色随临床分期逐渐增加，在晚期HCC中显示出非常强的染色。HCC中Wnt3a高表达的临床病理特征与低分化程度（χ(2)=20.211，P<0.001）、肝硬化（χ(2)=8.467，P<0.004）、乙型肝炎病毒（HBV）感染（χ(2)=12.957，P<0.001）、较高的肿瘤-淋巴结-转移分期（χ(2)=22.960，P<0.001）和5年生存率（χ(2)=15.469，P<0.001）有关。
与HBV感染和肝硬化肝脏相关的致癌性Wnt3a表达可能是HCC的一个独立预后因素。
