The Key Lab of Health Chemistry & Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering & Materials Science, Soochow University, Suzhou 215123, China.
Suzhou Industrial Park QingYuan Hong Kong & China Water Co., Ltd., Suzhou 215000, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2018 Feb 15;191:1-7. doi: 10.1016/j.saa.2017.09.068. Epub 2017 Sep 28.
A novel flow injection chemiluminescence immunoassay for simple, sensitive and low-cost detection of diclofenac was established based on specific binding of antigen and antibody. Carboxylic resin beads used as solid phase carrier materials provided good biocompatibility and large surface-to-volume ratio for modifying more coating antigen. There was a competitive process between the diclofenac in solution and the immobilized coating antigen to react with the limited binding sites of the polyclonal antibody to form the immunocomplex. The second antibody labelled with horseradish peroxidase was introduced into the immunosensor and trapped by captured polyclonal antibody against diclofenac, which could effectively amplify chemiluminescence signals of luminol-PIP-HO. Under optimal conditions, the diclofenac could be detected quantitatively. The chemiluminescence intensity decreased linearly with the logarithm of the diclofenac concentration in the range of 0.1-100ngmL with a detection limit of 0.05ngmL at a signal-to-noise ratio of 3. The immunosensor exhibited high sensitivity, specificity and acceptable stability. This easy-operated and cost-effective analytical method could be valuable for the diclofenac determination in real water samples.
基于抗原和抗体的特异性结合，建立了一种新颖的用于简单、灵敏和低成本检测双氯芬酸的流动注射化学发光免疫分析方法。羧酸树脂珠作为固相载体材料，为修饰更多的包被抗原提供了良好的生物相容性和大的表面积与体积比。在溶液中的双氯芬酸与固定化包被抗原之间存在竞争过程，与多克隆抗体的有限结合位点反应，形成免疫复合物。辣根过氧化物酶标记的第二抗体被引入免疫传感器中，并被针对双氯芬酸的捕获多克隆抗体捕获，这可以有效地放大鲁米诺-PIP-HO 的化学发光信号。在最佳条件下，可以定量检测双氯芬酸。在 0.1-100ngmL 的范围内，化学发光强度随双氯芬酸浓度的对数线性降低，信噪比为 3 时检测限为 0.05ngmL。该免疫传感器具有高灵敏度、特异性和可接受的稳定性。这种易于操作且具有成本效益的分析方法可用于实际水样中双氯芬酸的测定。
