Delannoy Yann, Colard Thomas, Cannet Catherine, Mesli Vadim, Hédouin Valéry, Penel Guillaume, Ludes Bertrand
Institut Médico-Légal de Paris, Université Paris Descartes Sorbonne Cité, 75000, Paris, France.
CHU de LILLE, 59000, Lille, France.
Int J Legal Med. 2018 Jan;132(1):219-227. doi: 10.1007/s00414-017-1699-y. Epub 2017 Sep 30.
The diagenesis of a bone in the postmortem period causes an identifiable deterioration in histology. This degradation is characterized by a collagenous alteration, which can be observed very early. In order to develop a method for determining a postmortem interval for medico-legal use, two ribs collected from six human bodies were studied prospectively over 2 years. Each bone was studied after staining with Sirius red to demonstrate the degradation of collagen as a function of time. This study demonstrated a time-based bone alteration characterized by the architectural degradation of the lamellar bone, without any microbial influence in this postmortem period. The staining was carried out by using Sirius red and correlated this alteration with a collagenic degradation by chemical hydrolysis owing to the affinity of this dye to the amino acids lysine, hydroxylysine, and arginine. Our work asserts that human bone samples that were studied in a controlled environment and analyzed for 24 months underwent a diagenetic trajectory whose main element was collagen hydrolysis.
死后时期骨骼的成岩作用会导致组织学上可识别的退化。这种退化的特征是胶原质改变，这在早期就能观察到。为了开发一种用于法医用途的死后间隔时间测定方法，前瞻性地对从六具人体采集的两根肋骨进行了为期两年的研究。每根骨头在用天狼星红染色后进行研究，以证明胶原质随时间的退化情况。这项研究表明，在这个死后时期，骨骼会出现基于时间的改变，其特征是板层骨的结构退化，且没有任何微生物影响。染色采用天狼星红进行，并由于这种染料对赖氨酸、羟赖氨酸和精氨酸的亲和力，将这种改变与化学水解导致的胶原质降解相关联。我们的研究表明，在受控环境中研究并分析24个月的人体骨骼样本经历了一个成岩过程，其主要因素是胶原质水解。
