Chen Xueli, Zhao Guanglu, Zhang Yang, Han Lujia, Xiao Weihua
Biomass and Bioresource Utilization Laboratory, College of Engineering, China Agricultural University , Beijing 100083, P.R. China.
J Agric Food Chem. 2017 Oct 25;65(42):9186-9190. doi: 10.1021/acs.jafc.7b03441. Epub 2017 Oct 12.
Accurately determining protein content is essential in exploiting biomass as feed and fuel. A survey of biomass samples in China indicated protein contents from 2.65 to 3.98% for crop residues and from 6.07 to 10.24% for animal manure of dry basis. Conversion factors based on amino acid nitrogen (k) ranged from 5.42 to 6.00 for the former and from 4.78 to 5.36 for the latter, indicating that the traditional factor of 6.25 is not suitable for biomass samples. On the other hand, conversion factors from Kjeldahl nitrogen (k) ranged from 3.97 to 4.57 and from 2.76 to 4.31 for crop residues and animal manure, respectively. Of note, conversion factors were strongly affected by amino acid composition and levels of nonprotein nitrogen. Thus, k values of 4.23 for crop residues, 4.11 for livestock manure, and 3.11 for poultry manure are recommended to better estimate protein content from total nitrogen.
准确测定蛋白质含量对于将生物质用作饲料和燃料至关重要。一项针对中国生物质样本的调查表明,以干基计,作物残渣的蛋白质含量为2.65%至3.98%,动物粪便的蛋白质含量为6.07%至10.24%。基于氨基酸氮的换算系数(k),前者范围为5.42至6.00,后者为4.78至5.36,这表明传统的6.25这个系数不适用于生物质样本。另一方面,凯氏氮的换算系数(k),作物残渣的范围为3.97至4.57,动物粪便的范围为2.76至4.31。值得注意的是,换算系数受氨基酸组成和非蛋白氮水平的影响很大。因此,建议分别采用4.23(作物残渣)、4.11(家畜粪便)和3.11(家禽粪便)的k值,以便从总氮中更好地估算蛋白质含量。