前列环素对大鼠左心室赖氨酸积累的抑制作用。

Prostacyclin-inhibition of lysine accumulation by the rat left ventricle.

Nasser M G, Marrush J, Nassar C F, Shammas N W, Jurjus A, Habbal M Z

Department of Physiology, Faculty of Medicine, American University of Beirut, Lebanon.

Comp Biochem Physiol A Comp Physiol. 1988;89(3):305-8. doi: 10.1016/0300-9629(88)91029-8.

Abstract

Left ventricular slices of male Sprague-Dawley rats were incubated with a fixed concentration of 0.5 microCi/ml 3H-lysine and several concentrations of unlabelled lysine ranging from 0.2 to 5.0 mM in control and prostacyclin-treated experiments. The time of incubation ranged from 0.5 to 90 min. 2. Left ventricular slices were cut to have an optimal thickness of 0.47 +/- 0.09 mm. 3. Lysine was taken up against a concentration gradient. Saturation was reached at 0.5 mM and steady state accumulation of lysine was attained within 60 min. 4. Prostacyclin in concentrations ranging from 1.2 x 10(-8) to 4.8 x 10(-8) M inhibited lysine transport in left ventricular slices significantly (P less than 0.01).

摘要

在对照实验和前列环素处理实验中，将雄性Sprague-Dawley大鼠的左心室切片与固定浓度为0.5微居里/毫升的³H-赖氨酸以及浓度范围为0.2至5.0毫摩尔的几种未标记赖氨酸一起孵育。孵育时间为0.5至90分钟。2. 将左心室切片切成最佳厚度0.47±0.09毫米。3. 赖氨酸逆浓度梯度摄取。在0.5毫摩尔时达到饱和，赖氨酸在60分钟内达到稳态积累。4. 浓度范围为1.2×10⁻⁸至4.8×10⁻⁸摩尔的前列环素显著抑制左心室切片中的赖氨酸转运（P<0.01）。