Jansen Christian H P, Brangsch Julia, Reimann Carolin, Adams Lisa, Hamm Bernd, Botnar Rene M, Makowski Marcus R
Division of Imaging Sciences, King's College London, London, United Kingdom.
Department of Radiology, Charité, Berlin, Germany.
Ultrasound Med Biol. 2017 Dec;43(12):2882-2890. doi: 10.1016/j.ultrasmedbio.2017.08.007. Epub 2017 Sep 28.
The development of abdominal aortic aneurysm (AAA) associated thrombi plays an important role during the onset and progression of AAAs. The aim of this study was to evaluate the potential of high-frequency ultrasound for characterization of AAA associated thrombi in an apolipoprotein-E-deficient mouse-model. Ultrasound measurements were performed using a high-resolution ultrasound system (Vevo770, FUJIFILM VisualSonics, Inc., Toronto, ON, Canada) with a 30 MHz linear-array transducer (RMV707 B). Magnetic resonance imaging with a 3 Tesla scanner (Achieva MR system, Philips Healthcare, Best, The Netherlands) and a single-loop microscopy coil was performed as a reference standard. All stages of aneurysm development were evaluated by histologic analyses. The "signal-thrombus-matrix" to "signal-blood" ratio on high-frequency ultrasound measurements showed a strong correlation (R = 0.81, p <0.05) with the state of extracellular matrix remodeling. Furthermore, size measurements derived from the high-frequency ultrasound correlated well with magnetic resonance imaging and histology. This study demonstrated that high-frequency ultrasound enables the reliable in vivo quantification of extracellular matrix remodeling at various stages of thrombus development, based on the thrombus echogenicity.
腹主动脉瘤（AAA）相关血栓的形成在AAA的发生和发展过程中起着重要作用。本研究的目的是评估高频超声在载脂蛋白E缺乏小鼠模型中对AAA相关血栓进行特征描述的潜力。使用配备30 MHz线性阵列换能器（RMV707 B）的高分辨率超声系统（Vevo770，富士胶片VisualSonics公司，加拿大多伦多）进行超声测量。使用3特斯拉扫描仪（Achieva MR系统，飞利浦医疗保健公司，荷兰贝斯特）和单环显微镜线圈进行磁共振成像作为参考标准。通过组织学分析评估动脉瘤发展的所有阶段。高频超声测量中“信号-血栓-基质”与“信号-血液”的比率与细胞外基质重塑状态显示出强烈的相关性（R = 0.81，p <0.05）。此外，高频超声得出的尺寸测量结果与磁共振成像和组织学结果相关性良好。本研究表明，基于血栓的回声性，高频超声能够在血栓发展的各个阶段对细胞外基质重塑进行可靠的体内定量。
