Makgoba M W, Sanders M E, Ginther Luce G E, Gugel E A, Dustin M L, Springer T A, Shaw S
Immunology Branch, National Cancer Institute, Bethesda.
Eur J Immunol. 1988 Apr;18(4):637-40. doi: 10.1002/eji.1830180423.
Although intercellular adhesion molecule-1 (ICAM-1) has been implicated as a ligand in some LFA-1-dependent adhesion, its importance to T cell function has not been established. The present studies investigate the importance of ICAM-1 for human cytotoxic T lymphocytes (CTL), both in their formation of antigen-independent conjugates (AIC) and in their lysis of targets. Analysis of monoclonal antibody (mAb) inhibition of AIC formation indicate that ICAM-1 mAb 1 blocks (a) AIC formation with some but not all targets; (b) the LFA-1 pathway but not the CD2/LFA-3 pathway of adhesion; (c) by binding to the target cell, not the T cell. In studies of cell-mediated lysis (CML) ICAM-1 mAb inhibited lysis of some targets, such as U-937, that use ICAM-1 predominantly in AIC formation; CML on some other targets is not inhibited by ICAM-1 mAb. These data indicate that ICAM-1 is a ligand for AIC formation, antigen-specific CTL recognition and cytolysis of particular target cells. The data also indicate that ICAM-1 is not used in LFA-1-dependent CTL interactions with all kinds of target cells, suggesting the existence of alternative ligands for LFA-1.
尽管细胞间黏附分子-1（ICAM-1）在某些依赖淋巴细胞功能相关抗原-1（LFA-1）的黏附中被认为是一种配体，但其对T细胞功能的重要性尚未确定。本研究探讨了ICAM-1对人细胞毒性T淋巴细胞（CTL）的重要性，包括其在抗原非依赖性结合物（AIC）形成以及对靶细胞的杀伤作用方面。对单克隆抗体（mAb）抑制AIC形成的分析表明，ICAM-1单克隆抗体1可阻断：（a）与部分而非全部靶细胞形成AIC；（b）黏附的LFA-1途径而非CD2/LFA-3途径；（c）通过与靶细胞而非T细胞结合。在细胞介导的杀伤作用（CML）研究中，ICAM-1单克隆抗体抑制了某些靶细胞（如U-937）的杀伤，这些靶细胞主要在AIC形成过程中使用ICAM-1；对其他一些靶细胞的CML不受ICAM-1单克隆抗体抑制。这些数据表明，ICAM-1是AIC形成、抗原特异性CTL识别以及特定靶细胞细胞溶解的配体。数据还表明，ICAM-1并非在LFA-1依赖的CTL与所有类型靶细胞的相互作用中都被使用，这表明存在LFA-1的替代配体。
