Gao Shan, Li Chunjin, Xu Ying, Chen Shuxiong, Zhao Yun, Chen Lu, Jiang Yanwen, Liu Zhuo, Fan Rong, Sun Liting, Wang Fengge, Zhu Xiaoling, Zhang Jing, Zhou Xu
College of Animal Sciences, Jilin University, Jilin, China.
Reproductive Medical Center, The Second Hospital of Jilin University, Changchun, China.
Cell Biochem Funct. 2017 Oct;35(7):364-371. doi: 10.1002/cbf.3283. Epub 2017 Oct 2.
Brain-derived neurotrophic factor (BDNF) is a neurotrophin that can promote the development and proliferation of neurons. BDNF has been found to be involved in male reproduction. Leydig cells in testicular interstitial tissues can secrete testosterone in a luteinizing hormone-dependent manner. We showed that BDNF and its receptor TrkB were expressed in mice TM3 Leydig cells in the present study. Furthermore, BDNF can promote proliferation of mouse TM3 Leydig cells in vitro. Results of microRNA (miRNA) deep sequencing showed that BDNF can alter the expression profile of miRNAs in TM3 Leydig cells. Eighty-three miRNAs were significantly different in the BDNF-treated and control groups (fold change of >2.0 or <0.5, P < 0.05) wherein 40 were upregulated and 43 were downregulated. The expression levels of miR-125a-5p, miR-22-5p, miR-342-59, miR-451a, miR-148a-5p, miR-29b-3p, miR-199b-5p, and miR-145a-5p were further confirmed by quantitative real-time polymerase chain reaction. Bioinformatic analysis revealed that miRNAs regulated a large number of genes with different functions. Pathway analysis indicated that miRNAs participate in the pathways involved in signal transduction, cancer, metabolism, endocrine system, immune system, and nerve system. This study indicated that miRNAs might be involved in the BDNF-regulated cellular functions of Leydig cells.
脑源性神经营养因子（BDNF）是一种神经营养蛋白，可促进神经元的发育和增殖。已发现BDNF与雄性生殖有关。睾丸间质组织中的睾丸间质细胞可通过促黄体生成素依赖的方式分泌睾酮。在本研究中，我们发现BDNF及其受体TrkB在小鼠TM3睾丸间质细胞中表达。此外，BDNF可在体外促进小鼠TM3睾丸间质细胞的增殖。微小RNA（miRNA）深度测序结果表明，BDNF可改变TM3睾丸间质细胞中miRNA的表达谱。在BDNF处理组和对照组中，83种miRNA存在显著差异（变化倍数>2.0或<0.5，P<0.05），其中40种上调，43种下调。通过定量实时聚合酶链反应进一步证实了miR-125a-5p、miR-22-5p、miR-342-59、miR-451a、miR-148a-5p、miR-29b-3p、miR-199b-5p和miR-145a-5p的表达水平。生物信息学分析显示，miRNA调控大量具有不同功能的基因。通路分析表明，miRNA参与信号转导、癌症、代谢、内分泌系统、免疫系统和神经系统相关的通路。本研究表明，miRNA可能参与BDNF调节的睾丸间质细胞的细胞功能。
