一种使用液相色谱-串联质谱法同时测定人唾液中尿酸和肌酐的灵敏且准确的方法。

A sensitive and accurate method to simultaneously measure uric acid and creatinine in human saliva by using LC-MS/MS.

作者信息

Liu Xin-Ying, Luo Ying, Zhou Chun-Yu, Peng Ai, Liu Jun-Yan

机构信息

Center for Nephrology & Metabolomics & Division of Nephrology & Rheumatology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.

出版信息

Bioanalysis. 2017 Nov;9(22):1751-1760. doi: 10.4155/bio-2017-0189. Epub 2017 Oct 2.

DOI:10.4155/bio-2017-0189

PMID:28967800

Abstract

AIM

To establish a method to simultaneously measure uric acid (UA) and creatinine (Cr) in human saliva.

MATERIALS & METHODS: By using HPLC-MS/MS, we developed and validated a fast, sensitive and accurate method to simultaneously determine UA and Cr in human saliva. The determination range for Cr and UA is of 10-5000 ng/ml with the R for both calibration curves over 0.999. The accuracy, precision and recovery of Cr and UA were all acceptable. By using the established method, the Cr and UA levels in saliva from 28 healthy volunteers were measured as 2.9 ± 0.8 µM and 46.8 ± 18.2 µM, respectively.

CONCLUSION

This method can simultaneously determine Cr and UA in saliva for clinical and translational study.

摘要

目的

建立一种同时测定人唾液中尿酸（UA）和肌酐（Cr）的方法。

材料与方法

通过高效液相色谱-串联质谱法（HPLC-MS/MS），我们开发并验证了一种快速、灵敏且准确的同时测定人唾液中UA和Cr的方法。Cr和UA的测定范围为10 - 5000 ng/ml，两条校准曲线的R均大于0.999。Cr和UA的准确度、精密度和回收率均可接受。使用所建立的方法，对28名健康志愿者唾液中的Cr和UA水平进行测定，结果分别为2.9±0.8 μM和46.8±18.2 μM。