Laboratory of Natural Medicines of the Changbai Mountain, Pharmaceutical Analysis, College of Pharmacy, Yanbian University, and Department of Pharmacy, Department of Endocrinology, Yanbian University Hospital, Yanji 133002, Jilin Province, China.
Laboratory of Natural Medicines of the Changbai Mountain, Pharmaceutical Analysis, College of Pharmacy, Yanbian University, and Department of Pharmacy, Department of Endocrinology, Yanbian University Hospital, Yanji 133002, Jilin Province, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2021 Feb 1;1164:122528. doi: 10.1016/j.jchromb.2021.122528. Epub 2021 Jan 6.
Concentration of uric acid (UA) in serum is one of the markers used to diagnose gout and hyperuricemia. However, serum treatment and storage are cumbersome, and wounds are susceptible to infection. Therefore, a new sampling and analysis method using noninvasive biological samples has been developed, called the dried spot method of UA in human saliva (DSM-UHS). Saliva (5 μL) was dropped on filter paper (a spot with a diameter of 5 mm) containing hypoxanthine (IS) (5 μL) and dried at room temperature for 30 min. The filter paper was immersed in 200 μL of lithium carbonate solution and shaken in a block bath shaker for 5 min at 30 °C. Afterward, the extraction was concentrated and reconstituted with 100 μL of lithium carbonate solution analyzed by HPLC-UV. When comparing the concentration of UA in the human saliva of hyperuricemia patients (HPs) and with that of healthy volunteers (HVs), we observed the concentration of UA was higher in the HPs than in the HVs (p < 0.0001). In addition, the results showed a significant linear relationship between the content of UA in saliva and the content of UA in the serum (r = 0.6243). The content of UA in human saliva could indirectly reflect the content of UA in human serum. Then DSM-UHS could be used to determine the content of UA in the saliva of HVs and HPs. This study provides a new research method and strategy for the determination of human UA content and the clinical prewiring of hyperuricemia.
血清中尿酸（UA）的浓度是用于诊断痛风和高尿酸血症的标志物之一。然而，血清处理和储存繁琐，伤口容易感染。因此，已经开发出一种使用非侵入性生物样本的新采样和分析方法，称为人唾液中尿酸的干式斑点法（DSM-UHS）。将 5μL 的唾液滴在含有次黄嘌呤（IS）的滤纸上（斑点直径为 5mm），在室温下干燥 30 分钟。将滤纸浸入 200μL 的碳酸锂溶液中，在 30°C 的块浴摇床上摇动 5 分钟。然后，用 HPLC-UV 分析浓缩和再构建 100μL 的碳酸锂溶液的提取物。在比较高尿酸血症患者（HPs）和健康志愿者（HVs）的人唾液中 UA 的浓度时，我们观察到 HPs 中的 UA 浓度高于 HVs（p<0.0001）。此外，结果表明唾液中 UA 的含量与血清中 UA 的含量之间存在显著的线性关系（r=0.6243）。人唾液中的 UA 含量可以间接反映人血清中的 UA 含量。然后可以使用 DSM-UHS 来确定 HVs 和 HPs 唾液中 UA 的含量。本研究为测定人 UA 含量和高尿酸血症的临床前期提供了一种新的研究方法和策略。
