Dorny P, Dermauw V, Van Hul A, Trevisan C, Gabriël S
Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium; Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium.
Vet Parasitol. 2017 Oct 15;245:39-41. doi: 10.1016/j.vetpar.2017.08.008. Epub 2017 Aug 18.
Taenia solium taeniasis/cysticercosis is a zoonosis included in the WHO's list of neglected tropical diseases. Accurate diagnostic tools for humans and pigs are needed to monitor intervention outcomes. Currently used diagnostic tools for porcine cysticercosis all have drawbacks. Serological tests are mainly confronted with problems of specificity. More specifically, circulating antigen detecting tests cross-react with Taenia hydatigena and the possibility of transient antigens as a result of aborted infections is suspected. Furthermore, the hypothesis has been raised that hatched ingested eggs of other Taenia species may lead to a transient antibody response or to the presence of circulating antigen detectable by serological tests used for porcine cysticercosis. Here we describe the results of a study that consisted of oral administration of Taenia saginata eggs to five piglets followed by serological testing during five weeks and necropsy aiming at studying possible cross reactions in serological tests used for porcine cysticercosis. The infectivity of the eggs was verified by in vitro hatching and by experimental infection of a calf. One piglet developed acute respiratory disease and died on day 6 post infection. The remaining four piglets did not show any clinical signs until euthanasia. None of the serum samples from four piglets collected between days 0 and 35 post infection gave a positive reaction in the B158/B60 Ag-ELISA and in a commercial Western blot for antibody detection. In conclusion, this study showed that experimental exposure of four pigs to T. saginata eggs did not result in positive serologies for T. solium. These results may help interpreting serological results in monitoring of T. solium control programmes.
猪带绦虫病/囊尾蚴病是一种人畜共患病，被列入世界卫生组织的被忽视热带病名单。需要准确的人类和猪诊断工具来监测干预效果。目前用于猪囊尾蚴病的诊断工具都有缺点。血清学检测主要面临特异性问题。更具体地说，循环抗原检测试验与泡状带绦虫存在交叉反应，并且怀疑流产感染会导致出现短暂抗原。此外，有人提出假说，即其他绦虫物种孵化的摄入卵可能导致短暂的抗体反应，或导致用于猪囊尾蚴病的血清学检测可检测到循环抗原的存在。在此，我们描述了一项研究的结果，该研究包括给5头仔猪口服牛带绦虫卵，随后在5周内进行血清学检测，并进行尸检，旨在研究用于猪囊尾蚴病的血清学检测中可能的交叉反应。通过体外孵化和对一头小牛进行实验性感染来验证卵的感染性。1头仔猪在感染后第6天出现急性呼吸道疾病并死亡。其余4头仔猪在安乐死之前未表现出任何临床症状。在感染后0至35天收集的4头仔猪的血清样本中，没有一份在B158/B60抗原酶联免疫吸附测定（Ag-ELISA）和用于抗体检测的商业蛋白质印迹法中呈阳性反应。总之，这项研究表明，4头猪实验性接触牛带绦虫卵并未导致猪带绦虫血清学检测呈阳性。这些结果可能有助于解释猪带绦虫控制项目监测中的血清学结果。
