Yücel Harun, Okumuşoğlu Nazmi Turan
Department of Physics, Faculty of Arts and Sciences, Ondokuz Mayıs University, 55139, Samsun, Turkey.
J Phys Condens Matter. 2017 Nov 22;29(46):465101. doi: 10.1088/1361-648X/aa908e.
In this paper, we propose a new algorithm based on radial symmetry center method to track colloidal particles close to contact, where the optical images of the particles start to overlap in digital video microscopy. This overlapping effect is important to observe the pair interaction potential in colloidal studies and it appears as additional interaction in the measurement of the interaction with conventional tracking analysis. The proposed algorithm in this work is simple, fast and applicable for not only two particles but also three and more particles without any modification. The algorithm uses gradient vectors of the particle intensity distribution, which allows us to use a part of the symmetric intensity distribution in the calculation of the actual particle position. In this study, simulations are performed to see the performance of the proposed algorithm for two and three particles, where the simulation images are generated by using fitted curve to experimental particle image for different sized particles. As a result, the algorithm yields the maximum error smaller than 2 nm for [Formula: see text] μm silica particles in contact condition.
在本文中，我们提出了一种基于径向对称中心法的新算法，用于追踪接近接触的胶体颗粒，在数字视频显微镜中，颗粒的光学图像开始重叠。这种重叠效应对于观察胶体研究中的对相互作用势很重要，并且在与传统跟踪分析的相互作用测量中表现为额外的相互作用。本文提出的算法简单、快速，不仅适用于两个颗粒，也适用于三个及更多颗粒，无需任何修改。该算法使用颗粒强度分布的梯度向量，这使我们能够在计算实际颗粒位置时使用对称强度分布的一部分。在本研究中，进行了模拟以观察所提出算法对两个和三个颗粒的性能，其中模拟图像是通过使用针对不同尺寸颗粒的实验颗粒图像的拟合曲线生成的。结果，对于处于接触状态的[公式：见原文]μm二氧化硅颗粒，该算法产生的最大误差小于2nm。
