Cabañas C, Sanchez-Madrid F, Acevedo A, Bellon T, Fernandez J M, Larraga V, Bernabeu C
Centro de Investigaciones Biológicas--C.S.I.C., Madrid, Spain.
Hybridoma. 1988 Apr;7(2):167-76. doi: 10.1089/hyb.1988.7.167.
A mouse monoclonal antibody (HC1/1) specific for a differentiation marker of human monocytes and granulocytes has been generated by using as immunogen the monocytic cell line U937 differentiated with phorbol esters. This differentiation antigen has been characterized as the p150/95 member of the CD11 family of glycoproteins by cellular distribution studies, immunoprecipitation and competition experiments with MAb 3.9 specific for the CD11c antigen. Immuno-alkaline phosphatase staining of normal tissue sections with the HC1/1 MAb demonstrated that the CD11c antigen is a useful macrophage marker. Furthermore, the MAb HC1/1 stains specifically populations of macrophages on skin and lymph node sections from different pathologies such as Sarcoidosis, Granuloma annulare, Lepromatous leprosy and Toxoplasmosis.
通过使用经佛波酯分化的单核细胞系U937作为免疫原，产生了一种针对人单核细胞和粒细胞分化标志物的小鼠单克隆抗体（HC1/1）。通过细胞分布研究、免疫沉淀以及与针对CD11c抗原的单克隆抗体3.9进行竞争实验，这种分化抗原已被鉴定为糖蛋白CD11家族的p150/95成员。用HC1/1单克隆抗体对正常组织切片进行免疫碱性磷酸酶染色表明，CD11c抗原是一种有用的巨噬细胞标志物。此外，单克隆抗体HC1/1可特异性地标记来自不同病理状况（如结节病、环状肉芽肿、瘤型麻风病和弓形虫病）的皮肤和淋巴结切片上的巨噬细胞群体。
