Lavinscky M P, Souza M M, Silva G S, Melo C A F
Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brasil.
Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, BA, Brasil
Genet Mol Res. 2017 Sep 27;16(3):gmr-16-03-gmr.16039582. doi: 10.4238/gmr16039582.
The analysis of meiotic behavior has been widely used in the study of plants as they provide relevant information about the viability of a species. Meiosis boasts a host of highly conserved events and changes in genes that control these events will give rise to irregularities that can alter the normal course of meiosis and may lead to complete sterility of the plant. The recombination of genes that occur in meiosis is an important event to generate variability and has been important in studies for genetic improvement and to create viable hybrids. The use of fluorescence in situ hybridization and genomic in situ hybridization (GISH) in meiosis allows the localization of specific regions, enables to differentiate genomes in a hybrid, permits to observe the pairing of homoeologous chromosomes, and if there was a recombination between the genomes of progenitor species. Furthermore, the GISH allows us to observe the close relationship between the species involved. This article aims to report over meiosis studies on plants and hybrids, the use and importance of molecular cytogenetic in meiotic analysis and contributions of meiotic analysis in breeding programs.
减数分裂行为分析在植物研究中已被广泛应用,因为它们能提供有关物种生存能力的相关信息。减数分裂拥有许多高度保守的事件,控制这些事件的基因发生变化会导致不规则现象,从而改变减数分裂的正常进程,并可能导致植物完全不育。减数分裂过程中发生的基因重组是产生变异性的重要事件,在遗传改良研究和培育可行杂种方面一直很重要。在减数分裂中使用荧光原位杂交和基因组原位杂交(GISH)可以定位特定区域,能够区分杂种中的基因组,允许观察同源染色体的配对,以及祖先物种基因组之间是否发生了重组。此外,GISH使我们能够观察所涉及物种之间的密切关系。本文旨在报告关于植物和杂种的减数分裂研究、分子细胞遗传学在减数分裂分析中的应用和重要性以及减数分裂分析在育种计划中的贡献。