Ma S J, Sa K J, Hong T K, Lee J K
Department of Applied Plant Sciences, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Korea.
Department of Applied Plant Sciences, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon, Korea
Genet Mol Res. 2017 Sep 21;16(3):gmr-16-03-gmr.16039746. doi: 10.4238/gmr16039746.
In this study, 21 simple sequence repeat (SSR) markers were used to evaluate the genetic diversity and population structure among 77 Perilla accessions from high-latitude and middle-latitude areas of China. Ninety-five alleles were identified with an average of 4.52 alleles per locus. The average polymorphic information content (PIC) and genetic diversity values were 0.346 and 0.372, respectively. The level of genetic diversity and PIC value for cultivated accessions of Perilla frutescens var. frutescens from middle-latitude areas were higher than accessions from high-latitude areas. Based on the dendrogram of unweighted pair group method with arithmetic mean (UPGMA), all accessions were classified into four major groups with a genetic similarity of 46%. All accessions of the cultivated var. frutescens were discriminated from the cultivated P. frutescens var. crispa. Furthermore, most accessions of the cultivated var. frutescens collected in high-latitude and middle-latitude areas were distinguished depending on their geographical location. However, the geographical locations of several accessions of the cultivated var. frutescens have no relation with their positions in the UPGMA dendrogram and population structure. This result implies that the diffusion of accessions of the cultivated Perilla crop in the northern areas of China might be through multiple routes. On the population structure analysis, 77 Perilla accessions were divided into Group I, Group II, and an admixed group based on a membership probability threshold of 0.8. Finally, the findings in this study can provide useful theoretical knowledge for further study on the population structure and genetic diversity of Perilla and benefit for Perilla crop breeding and germplasm conservation.
在本研究中，使用21个简单序列重复（SSR）标记评估了来自中国高纬度和中纬度地区的77份紫苏种质的遗传多样性和群体结构。共鉴定出95个等位基因，每个位点平均有4.52个等位基因。平均多态信息含量（PIC）和遗传多样性值分别为0.346和0.372。中纬度地区栽培的紫苏变种紫苏的遗传多样性水平和PIC值高于高纬度地区的种质。基于非加权算术平均法（UPGMA）的聚类图，所有种质被分为四个主要类群，遗传相似性为46%。所有栽培的紫苏变种均与栽培的皱叶紫苏区分开来。此外，在高纬度和中纬度地区收集的大多数栽培紫苏变种种质根据其地理位置得以区分。然而，几个栽培紫苏变种种质的地理位置与其在UPGMA聚类图中的位置和群体结构无关。这一结果表明，中国北方地区栽培紫苏作物种质的扩散可能是通过多种途径。在群体结构分析中，根据隶属概率阈值0.8，将77份紫苏种质分为第一组、第二组和一个混合组。最后，本研究的结果可为进一步研究紫苏的群体结构和遗传多样性提供有用的理论知识，并有助于紫苏作物育种和种质保存。
