Silva R A R, Fajardo C G, Vieira F A
Unidade Acadêmica Especializada em Ciências Agrárias, Universidade Federal do Rio Grande do Norte, Macaíba, RN, Brasil
Unidade Acadêmica Especializada em Ciências Agrárias, Universidade Federal do Rio Grande do Norte, Macaíba, RN, Brasil.
Genet Mol Res. 2017 Sep 21;16(3):gmr-16-03-gmr.16039764. doi: 10.4238/gmr16039764.
The aim of this study was to investigate the mating system and genetic diversity of the palm Copernicia prunifera using inter-simple sequence repeat markers. We found that the C. prunifera has multiple inflorescences with hermaphroditic flowers and pollen viability of 62%. Outcrossing rates at the population level (N = 267) produced a multilocus outcrossing rate (t) of 0.878 and a single-locus outcrossing rate (t) of 0.738, indicating that C. prunifera has a mixed mating system that is preferentially allogamous. The rate of mating among relatives (t - t) was low, indicating limited outcrossing between closely related individuals. The fixation index between seed trees (F) was negative (0.200), suggesting an absence of inbreeding, while the correlation of selfing (r) was high (0.914). The values of the diversity indices among adults and progenies did not differ statistically (H = 0.319 and I = 0.470; H = 0.337 and I = 0.505, respectively). In testing for the presence of genetic bottlenecks using the infinite allele model and the stepwise mutation model, we observed a reduction in the effective population, as well as a deficit in heterozygosity (P < 0.0001). The results of this study inform management strategies for the conservation and genetic improvement of the C. prunifera palm.
本研究的目的是利用简单序列重复区间标记来调查蒲葵的交配系统和遗传多样性。我们发现，蒲葵有多个带有两性花的花序，花粉活力为62%。在种群水平（N = 267）上的异交率产生了一个多位点异交率（t）为0.878，单位点异交率（t）为0.738，这表明蒲葵具有一种优先异交的混合交配系统。近亲之间的交配率（t - t）较低，表明亲缘关系较近的个体之间异交有限。母树之间的固定指数（F）为负（0.200），表明不存在近亲繁殖，而自交相关性（r）较高（0.914）。成年个体和子代之间的多样性指数值在统计学上没有差异（分别为H = 0.319和I = 0.470；H = 0.337和I = 0.505）。在使用无限等位基因模型和逐步突变模型检测遗传瓶颈的存在时，我们观察到有效种群减少，以及杂合性不足（P < 0.0001）。本研究结果为蒲葵的保护和遗传改良管理策略提供了依据。
