Ferreira R R, Santos C A F, Oliveira V R
Programa de Pós-Graduação em Agronomia - Melhoramento Genético de Plantas, , , Brasil
, , Brasil.
Genet Mol Res. 2017 Sep 21;16(3):gmr-16-03-gmr.16039766. doi: 10.4238/gmr16039766.
The objective of this study was the identification of the cytoplasmic types and the genotyping for the fertility restoration nuclear locus (Ms) in 59 onion accessions, aiming at the selection of 'A' and 'B' lines essential for the obtainment of hybrids. Three markers were used to identify the cytoplasm 5' cob, orfA501, and orf725, and two were used for the Ms locus (AcSKP1 and AcPMS1). The two types of male-sterile cytoplasm ('S' and 'T'), as well as fertile cytoplasm ('N'), and the Ms and ms alleles in both homozygosity and heterozygosity were detected in the 59 genotypes evaluated in the experiment. The frequencies of the 5' cob/orfA501 and orf725 markers, as well as of the markers AcSKP1 and AcPMS1, were close in the onion accessions evaluated in this study. In the Brazilian germplasm, the frequencies of the 'N', 'S', and 'T' cytoplasm were approximately 0.47, 0.28, and 0.25, respectively, whereas the allele frequencies of Ms and ms were 0.52 and 0.48, respectively. The accessions Régia, EHCEB 20146, EHCEB 201427, Alvorada, Serrana, Crioula Mercosul, EHCEB 20142, BRS 367, Rainha, Juporanga, and Alfa SF C-XI have potential for the identification of 'A' and 'B' lines, since they presented mixtures of cytoplasm and different allele frequencies for Ms. All the plants of the accessions EHCEB 20142040/EHCEB 20141040, EHCEB 20142028/EHCEB 20141028, and EHCEB 20112006/EHCEB 20111006 were in the Nmsms and Smsms conditions, and have the potential for 'B' and 'A' lines, respectively, for the CMS-S system. All the plants of the accessions EHCEB 20142027/EHCEB 20141027, EHCEB 20102019/EHCEB 20101019, and Alfa SF 'B'/Alfa SF 'A' were in Nmsms and Tmsms conditions, and have the potential for 'B' and 'A' lines, respectively, for the CMS-T system.
本研究的目的是鉴定59份洋葱种质的细胞质类型，并对育性恢复核基因座（Ms）进行基因分型，旨在选择获得杂交种所必需的“A”系和“B”系。使用三个标记来鉴定细胞质5' cob、orfA501和orf725，两个标记用于Ms基因座（AcSKP1和AcPMS1）。在本实验评估的59个基因型中，检测到了两种雄性不育细胞质类型（“S”和“T”）以及可育细胞质（“N”），以及纯合和杂合状态的Ms和ms等位基因。在本研究评估的洋葱种质中，5' cob/orfA501和orf725标记以及AcSKP1和AcPMS1标记的频率相近。在巴西种质中，“N”、“S”和“T”细胞质的频率分别约为0.47、0.28和0.25，而Ms和ms的等位基因频率分别为0.52和0.48。Régia、EHCEB 20146、EHCEB 201427、Alvorada、Serrana、Crioula Mercosul、EHCEB 20142、BRS 367、Rainha、Juporanga和Alfa SF C-XI这些种质具有鉴定“A”系和“B”系的潜力，因为它们呈现出细胞质的混合以及Ms的不同等位基因频率。EHCEB 20142040/EHCEB 20141040、EHCEB 20142028/EHCEB 20141028和EHCEB 20112006/EHCEB 20111006这些种质的所有植株分别处于Nmsms和Smsms状态，对于CMS-S系统分别具有作为“B”系和“A”系的潜力。EHCEB 20142027/EHCEB 20141027、EHCEB 20102019/EHCEB 20101019和Alfa SF 'B'/Alfa SF 'A'这些种质的所有植株分别处于Nmsms和Tmsms状态，对于CMS-T系统分别具有作为“B”系和“A”系的潜力。
