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外源生长素通过降低赤霉素/脱落酸（GA/ABA）比值来抑制大豆种子的萌发。

Exogenous auxin represses soybean seed germination through decreasing the gibberellin/abscisic acid (GA/ABA) ratio.

机构信息

Key Laboratory of Crop Ecophysiology and Farming System in Southwest China (Ministry of Agriculture), Sichuan Engineering Research Center for Crop Strip Intercropping System, Institute of Ecological Agriculture, Sichuan Agricultural University, Chengdu, 611130, China.

出版信息

Sci Rep. 2017 Oct 3;7(1):12620. doi: 10.1038/s41598-017-13093-w.

DOI:10.1038/s41598-017-13093-w
PMID:28974733
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5626727/
Abstract

Auxin is an important phytohormone which mediates diverse development processes in plants. Published research has demonstrated that auxin induces seed dormancy. However, the precise mechanisms underlying the effect of auxin on seed germination need further investigation, especially the relationship between auxins and both abscisic acid (ABA) and gibberellins (GAs), the latter two phytohormones being the key regulators of seed germination. Here we report that exogenous auxin treatment represses soybean seed germination by enhancing ABA biosynthesis, while impairing GA biogenesis, and finally decreasing GA/ABA and GA/ABA ratios. Microscope observation showed that auxin treatment delayed rupture of the soybean seed coat and radicle protrusion. qPCR assay revealed that transcription of the genes involved in ABA biosynthetic pathway was up-regulated by application of auxin, while expression of genes involved in GA biosynthetic pathway was down-regulated. Accordingly, further phytohormone quantification shows that auxin significantly increased ABA content, whereas the active GA and GA levels were decreased, resulting insignificant decreases in the ratiosGA/ABA and GA/ABA.Consistent with this, ABA biosynthesis inhibitor fluridone reversed the delayed-germination phenotype associated with auxin treatment, while paclobutrazol, a GA biosynthesis inhibitor, inhibited soybean seed germination. Altogether, exogenous auxin represses soybean seed germination by mediating ABA and GA biosynthesis.

摘要

生长素是一种重要的植物激素，介导植物的多种发育过程。已发表的研究表明，生长素诱导种子休眠。然而，生长素对种子萌发影响的确切机制需要进一步研究，特别是生长素与脱落酸（ABA）和赤霉素（GAs）之间的关系，后两者是种子萌发的关键调节剂。在这里，我们报告说，外源生长素处理通过增强 ABA 生物合成、破坏 GA 生物合成来抑制大豆种子萌发，最终降低 GA/ABA 和 GA/ABA 比值。显微镜观察表明，生长素处理延迟了大豆种皮破裂和胚根突出。qPCR 分析显示，生长素处理上调了参与 ABA 生物合成途径的基因的转录，而下调了参与 GA 生物合成途径的基因的表达。因此，进一步的植物激素定量分析表明，生长素显著增加了 ABA 含量，而活性 GA 和 GA 水平降低，导致 GA/ABA 和 GA/ABA 比值无明显降低。与此一致的是，ABA 生物合成抑制剂 fluridone 逆转了与生长素处理相关的延迟发芽表型，而 GA 生物合成抑制剂 paclobutrazol 抑制了大豆种子的萌发。总之，外源生长素通过调节 ABA 和 GA 生物合成来抑制大豆种子萌发。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f71d/5626727/2ebe69d51858/41598_2017_13093_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f71d/5626727/c3ee1ae46bf3/41598_2017_13093_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f71d/5626727/222ed4ed8164/41598_2017_13093_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f71d/5626727/c98e4e791568/41598_2017_13093_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f71d/5626727/25f0580d7b37/41598_2017_13093_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f71d/5626727/aa7fe31ea4ea/41598_2017_13093_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f71d/5626727/2ebe69d51858/41598_2017_13093_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f71d/5626727/c3ee1ae46bf3/41598_2017_13093_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f71d/5626727/222ed4ed8164/41598_2017_13093_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f71d/5626727/c98e4e791568/41598_2017_13093_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f71d/5626727/25f0580d7b37/41598_2017_13093_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f71d/5626727/aa7fe31ea4ea/41598_2017_13093_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f71d/5626727/2ebe69d51858/41598_2017_13093_Fig6_HTML.jpg

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