Department of Orthopedics, Shuyang Hospital of Traditional Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Suqian, China.
Eur Rev Med Pharmacol Sci. 2017 Oct;21(17):3754-3762.
The dysregulation of proliferation and apoptosis plays a significant role in the pathogenesis of hormone-induced osteonecrosis of femoral head (ONFH). The research aimed to explore the regulatory role of miR-146a in dexamethasone (DEX)-induced proliferation and apoptosis change in MC3T3-E1 cells from murine osteoblastic.
In this study, MC3T3-E1 was co-cultured with 10-7 DEX for 6 h, then RT-PCR was employed to test the expression level of miR-146a and Bcl2. CCK8 assay and flow cytometry were adopted to verify miR-146a could regulate proliferation and apoptosis. After transfected MC3T3-E1 with mimics and inhibitor, RT-PCR and Western blot was used to detect Bcl2 expression level.
In DEX treated MC3T3-E1 cells showed higher MiR-146a expression level and lower Bcl2 expression level. MiR-146a could inhibit proliferation and promotes apoptosis in murine osteoblastic MC3T3-E1 cells. Additionally, Bcl2 gene is regulated by MiR-146a.
The MiR-146a expression level increased, while Bcl2 has low expression level in dexamethasone treated MC3T3-E1 cells. MiR-146a regulates proliferation and apoptosis of mouse bone cells. The low expression level of Bcl2 in DEX treated MC3T3-E1 cells is caused by increased MiR-146a level.
增殖和凋亡失调在激素诱导性股骨头坏死（ONFH）的发病机制中起重要作用。本研究旨在探讨 miR-146a 在糖皮质激素（DEX）诱导的鼠成骨细胞 MC3T3-E1 增殖和凋亡变化中的调节作用。
本研究中，MC3T3-E1 与 10-7 DEX 共培养 6 小时，然后采用 RT-PCR 检测 miR-146a 和 Bcl2 的表达水平。CCK8 检测和流式细胞术用于验证 miR-146a 可调节增殖和凋亡。用 mimics 和抑制剂转染 MC3T3-E1 后，采用 RT-PCR 和 Western blot 检测 Bcl2 表达水平。
DEX 处理的 MC3T3-E1 细胞中 MiR-146a 表达水平升高，Bcl2 表达水平降低。MiR-146a 可抑制鼠成骨细胞 MC3T3-E1 的增殖并促进其凋亡。此外，Bcl2 基因受 MiR-146a 调控。
DEX 处理的 MC3T3-E1 细胞中 MiR-146a 表达水平升高，Bcl2 表达水平降低。MiR-146a 调节鼠成骨细胞的增殖和凋亡。DEX 处理的 MC3T3-E1 细胞中 Bcl2 表达水平降低是由于 MiR-146a 水平升高所致。
