Davidson S E, McKenzie J L, Beard M E, Hart D N
Department of Haematology, Christchurch Hospital, New Zealand.
Pathology. 1988 Jan;20(1):24-31. doi: 10.3109/00313028809085192.
Two monoclonal antibodies, CMRF-7 and 27, which react with cells of the granulocytic series, were obtained from hybridomas cloned from separate fusions. Biochemical studies indicate that both antibodies are of the CD15 group and react with the antigenic determinant 3 alpha-fucosyl-N-acetyl lactosamine (hapten X) expressed on some glycolipids and several different granulocyte glycoproteins with a wide range of molecular weights. The antigen was found on some promyelocytes and more differentiated granulocytes, including neutrophils and some eosinophils, but not basophils. Monocytes, lymphocytes, and erythrocytes were negative for CMRF-7 but neuraminidase treatment revealed "cryptic" sites on monocytes and some lymphoid cells. The antibody CMRF-7 reacted with the majority of acute myeloid leukemia blasts in the FAB categories M2-M5 but less frequently with M1 blasts and was positive with only 5/43 acute lymphoid leukemias. Immunoperoxidase staining of other normal human tissues indicates that this determinant is found on a range of epithelial cells in skin, the gastrointestinal tract and the genitourinary system. In addition some parts of the central nervous tissue and some endocrine organs stained with these antibodies.
从不同融合克隆得到的杂交瘤中获得了两种与粒细胞系细胞发生反应的单克隆抗体，即CMRF - 7和27。生化研究表明，这两种抗体均属于CD15组，与某些糖脂和几种不同分子量的粒细胞糖蛋白上表达的抗原决定簇3α - 岩藻糖基 - N - 乙酰乳糖胺（半抗原X）发生反应。该抗原存在于一些早幼粒细胞和更分化的粒细胞中，包括中性粒细胞和一些嗜酸性粒细胞，但不存在于嗜碱性粒细胞中。单核细胞、淋巴细胞和红细胞对CMRF - 7呈阴性反应，但神经氨酸酶处理后在单核细胞和一些淋巴细胞上显示出“隐蔽”位点。抗体CMRF - 7与FAB分类为M2 - M5的大多数急性髓细胞白血病原始细胞发生反应，但与M1原始细胞反应较少，并且仅在43例急性淋巴细胞白血病中有5例呈阳性。对其他正常人体组织进行免疫过氧化物酶染色表明，在皮肤、胃肠道和泌尿生殖系统的一系列上皮细胞中发现了该决定簇。此外，中枢神经组织的一些部分和一些内分泌器官也被这些抗体染色。
