Lei Mingdao, Gan Wei, Sun Yongbing
Department of Pharmacy, Jiangxi Maternal and Child Health Hospital, Nanchang, China.
Division of Pharmaceutics, National Pharmaceutical Engineering Center for Solid Preparation in Chinese Herbal Medicine, Jiangxi University of Traditional Chinese Medicine, Nanchang, China.
Biomed Chromatogr. 2018 Mar;32(3). doi: 10.1002/bmc.4103. Epub 2017 Oct 25.
A simple HPLC-MS/MS method has been developed for the determination of peramivir in rat plasma in the present study. The analytes were separated on a C column (50 × 2.1 mm, 1.7 μm) and a triple-quadrupole mass spectrometer equipped with an electrospray ionization source was applied for the detection. A phospholipid-free cartridge solid-phase extraction was used to pretreat the plasma and eliminate the endogenous phospholipid. The in-source collision-induced dissociation approach showed that this pretreatment could result in negligible ion suppression from the extracted sample and could produce cleaner samples when compared with the protein precipitation. The method was linear over the concentration range of 0.12-1200.0 ng/mL for peramivir. The method was validated and successfully applied to a pharmacokinetic study after peramivir was orally and intravenously administered to Sprague-Dawley rats.
在本研究中，已开发出一种简单的高效液相色谱-串联质谱法用于测定大鼠血浆中的帕拉米韦。分析物在C柱（50×2.1 mm，1.7μm）上分离，并使用配备电喷雾电离源的三重四极杆质谱仪进行检测。采用无磷脂柱固相萃取法对血浆进行预处理，以消除内源性磷脂。源内碰撞诱导解离方法表明，与蛋白质沉淀法相比，这种预处理可使提取样品中的离子抑制作用降至可忽略不计，并能产生更纯净的样品。该方法在帕拉米韦浓度范围为0.12-1200.0 ng/mL时呈线性。该方法经过验证，并成功应用于对Sprague-Dawley大鼠口服和静脉注射帕拉米韦后的药代动力学研究。
