Department of Chemical Engineering and ‡Department of Biomedical Engineering, Worcester Polytechnic Institute , 100 Institute Road, Worcester, Massachusetts 01609, United States.
Langmuir. 2018 Jan 23;34(3):1178-1189. doi: 10.1021/acs.langmuir.7b02846. Epub 2017 Oct 17.
Polyelectrolyte multilayers (PEMs) are of great interest as cell culture surfaces because of their ability to modify topography and surface energy and release biologically relevant molecules such as growth factors. In this work, fibroblast growth factor 2 (FGF2) was adsorbed directly onto polystyrene, plasma-treated polystyrene, and glass surfaces with a poly(methacrylic acid) and poly-l-histidine PEM assembled above it. Up to 14 ng/cm of FGF2 could be released from plasma-treated polystyrene surfaces over the course of 7 days with an FGF2 solution concentration of 100 μg/mL applied during the adsorption process. This release rate could be modulated by adjusting the adsorption concentration, decreasing to as low as 2 ng/cm total release over 7 days using a 12.5 μg/mL FGF2 solution. The surface energy and roughness could also be regulated using the adsorbed PEM. These properties were found to be substrate- and first-layer-dependent, supporting current theories of PEM assembly. When released, FGF2 from the PEMs was found to significantly enhance fibroblast proliferation as compared to culture conditions without FGF2. The results showed that growth factor release profiles and surface properties are easily controllable through modification of the PEM assembly steps and that these strategies can be effectively applied to common cell culture surfaces to control the cell fate.
聚电解质多层（PEMs）作为细胞培养表面具有很大的吸引力，因为它们能够改变形貌和表面能，并释放生物相关分子，如生长因子。在这项工作中，直接将成纤维细胞生长因子 2（FGF2）吸附到聚苯乙烯、等离子体处理的聚苯乙烯和玻璃表面上，然后在其上方组装一层聚（甲基丙烯酸）和聚-L-组氨酸 PEM。在吸附过程中，将浓度为 100 μg/mL 的 FGF2 溶液施加到等离子体处理的聚苯乙烯表面上，可以在 7 天内释放多达 14 ng/cm 的 FGF2。通过调整吸附浓度，可以调节这种释放速率，使用浓度为 12.5 μg/mL 的 FGF2 溶液，总释放量可低至 7 天内 2 ng/cm。还可以通过吸附的 PEM 来调节表面能和粗糙度。这些性质取决于基底和第一层，支持 PEM 组装的当前理论。当从 PEM 中释放时，与没有 FGF2 的培养条件相比，FGF2 显著促进了成纤维细胞的增殖。结果表明，通过修改 PEM 组装步骤可以轻松控制生长因子的释放曲线和表面性质，并且这些策略可以有效地应用于常见的细胞培养表面来控制细胞命运。
