Hsu Ai-Ping, Tseng Chun-Hsien, Barrat Jacques, Lee Shu-Hwae, Shih Yu-Hua, Wasniewski Marine, Mähl Philippe, Chang Chia-Chia, Lin Chun-Ta, Chen Re-Shang, Tu Wen-Jane, Cliquet Florence, Tsai Hsiang-Jung
Division of Biologics, Animal Health Research Institute, Council of Agriculture, New Taipei City, Taiwan.
Graduate Institute of Veterinary Medicine, School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan.
PLoS One. 2017 Oct 4;12(10):e0184831. doi: 10.1371/journal.pone.0184831. eCollection 2017.
Since 2013, rabies cases have been reported among Formosan ferret badgers in Taiwan, and they have been shown to be the major reservoirs for Taiwanese enzootics. To control and eradicate rabies, the authorities plan to implement a vaccination programme. Before distributing live vaccines in the field, this study assessed the safety, efficacy, and immunogenicity of SAG2 vaccine on ferret badgers by direct oral instillation. After application of 109 TCID50/dose, no virus was excreted into the oral cavity 1-7 days post-application, and safety was also satisfactorily verified over a 266-day period. Moreover, despite the low level of rabies virus neutralising antibodies induced after vaccination of a 108 TCID50/dose, the efficacy assessment revealed a 100% survival rate (15/15) of vaccinees and an 87.5% fatality rate (7/8) in control animals after a challenge on the 198th day post-vaccination. The immunisation and protection rates obtained more than 6 months after a single vaccination dose demonstrated that SAG2 is an ideal vaccine candidate to protect Formosan ferret badgers against rabies in Taiwan.
自2013年以来，台湾已报告在台湾鼬獾中出现狂犬病病例，并且它们已被证明是台湾地方性动物病的主要宿主。为了控制和根除狂犬病，当局计划实施一项疫苗接种计划。在野外分发活疫苗之前，本研究通过直接口服滴注评估了SAG2疫苗对鼬獾的安全性、有效性和免疫原性。在应用109 TCID50/剂量后，在应用后1-7天内没有病毒排泄到口腔中，并且在266天的时间内安全性也得到了令人满意的验证。此外，尽管在接种108 TCID50/剂量后诱导的狂犬病病毒中和抗体水平较低，但疗效评估显示，在接种后第198天进行攻击后，疫苗接种者的存活率为100%（15/15），对照动物的死亡率为87.5%（7/8）。单次接种剂量6个月以上获得的免疫和保护率表明，SAG2是保护台湾鼬獾免受狂犬病侵害的理想候选疫苗。
