Riisom K, Søorensen I J, Møoller B, Kruse T A, Graugaard B, Lamm L U
Department of Clinical Immunology, Aarhus University Hospital, Denmark.
Tissue Antigens. 1988 Mar;31(3):141-50. doi: 10.1111/j.1399-0039.1988.tb02075.x.
A panel of 43 homozygous and 36 heterozygous highly selected cells, representing the most common DR-specificities, were investigated with the DNA hybridization technique. By using a single restriction enzyme, TaqI, and two probes, DR beta and DQ alpha, it was possible to construct assignment criteria giving a reasonable definition of DR1, 3, 4, 5, 7 + w9, w8, w10 and w11. The criteria sometimes require that certain bands must not be present. Therefore, in certain genotypic combinations, the presence or absence of the particular specificity on one haplotype cannot be decided. This is a problem only for DR2 and DRw6, which for this reason cannot be assigned in about 1/3 to 1/4 of the cases. The association between RFLP assignment and serological assignment is not absolute, the correlation coefficients ranking from 0.62 to 1.0. In the case of false negative RFLP assignment, this may be due to genetic heterogeneity, as in the case of a DR2 individual who proved to be Dw12 and not Dw2 associated. It is often stated that interpretation of the RFLP pattern is particularly difficult in random or heterozygous individuals compared to proven homozygotes. This is not the case in the present study, where in fact correlation coefficients between RFLP and serologically determined DR specificities were higher in the heterozygotes (range 0.79-1.00).
利用DNA杂交技术对一组43个纯合子和36个杂合子的高度选择细胞进行了研究，这些细胞代表了最常见的DR特异性。通过使用单一限制性内切酶TaqI和两个探针DRβ和DQα，有可能构建分配标准，从而对DR1、3、4、5、7+w9、w8、w10和w11给出合理的定义。这些标准有时要求某些条带不能出现。因此，在某些基因型组合中，一个单倍型上特定特异性的存在与否无法确定。这只是DR2和DRw6的一个问题，因此在大约1/3到1/4的病例中无法进行分配。RFLP分配与血清学分配之间的关联不是绝对的，相关系数在0.62至1.0之间。在RFLP分配出现假阴性的情况下，这可能是由于遗传异质性，例如一个DR2个体被证明是Dw12，而不是与Dw2相关。人们常说，与已证实的纯合子相比，在随机或杂合子个体中对RFLP模式的解释特别困难。在本研究中并非如此，实际上杂合子中RFLP与血清学确定的DR特异性之间的相关系数更高（范围为0.79-1.00）。
