The Optimized HPLC Method for Quantitative Analysis of Phenylethyl Resorcinol Loaded in the Novel Vesicle Carriers and Permeated in In Vitro Skin Permeation Study.

The high performance liquid chromatography (HPLC) was used for quantitative determination of phenylethyl resorcinol (PR) which was loaded in the novel vesicle carriers including ethosome, invasome and transfersome formulations, and permeated into pig skin membrane and receptor fluid for skin permeation study. The reverse-phase chromatography was carried out with a C18 column (150 × 4.6 mm2, 5 μm, HypersilTM, Thermo Fisher Scientific Inc, USA) with the column temperature at 25°C. A mixture of acetonitrile-methanol-Milli-Q water in the ratio of 40:20:40%, v/v/v was used as a mobile phase by maintaining the flow rate at 0.8 mL/min. The 20 μL sample solution was injected and the absorbance was detected at 254 nm using an HPLC Agilent 1100 series. This method gave the chromatogram with symmetric peak of PR at the appropriate retention time of 4.620 min. At such retention time no interfering peaks were detected from other matrix components. All %recovery and %RSD values of PR analysis were in the range of 98-102% and not more than 2.0%, respectively. From the validation data, the method demonstrated that it had satisfactory specificity, sensitivity, linearity, accuracy and precision appropriate for analysis of PR in the presence of vesicle carriers and skin permeation study.