Wang Meng, Cheng Chunsheng, Liu Chunbo, Yang Yaling
Faculty of Life Science and Technology, Kunming University of Science and Technology, No. 727, Jingming South Road, Chenggong District, Yunnan Province, Kunming 650500, China.
Yunnan Province Food Safety Research Institute, Kunming University of Science and Technology, No. 727, Jingming South Road, Chenggong District, Yunnan Province, Kunming 650500, China.
J Chromatogr Sci. 2018 Jan 1;56(1):74-80. doi: 10.1093/chromsci/bmx075.
A rapid, simple, reliable and efficient hollow fiber supported ionic liquids liquid-phase micro-extraction method (IL-HF-LPME) followed by high-performance liquid chromatography was successfully applied to the determination of four kinds of polycyclic aromatic hydrocarbons (PAHs) in milk samples. In the IL-HF-LPME method, a mixture of [OMIM]PF6 and lauric acid, in a ratio of 3:1, was immobilized in the pores of a polypropylene hollow fiber used as extraction solvent. A series of essential parameters influencing the extraction efficiency were investigated and optimized. Under the optimal conditions, the extraction equilibrium is achieved within 3 min, the good linearity was >0.9990, the limits of detection varied from 0.14 to 0.71 ng/mL, the limit of quantification values were between 0.4 and 1.8 ng/mL, and the relative standard deviations were in the range of 1.24-3.27% (n = 5). The proposed method was applied to analyze four PAHs in milk samples and recoveries were between 93.6 and 102.8%.
一种快速、简单、可靠且高效的中空纤维支撑离子液体液相微萃取法（IL-HF-LPME），随后结合高效液相色谱法，成功应用于牛奶样品中四种多环芳烃（PAHs）的测定。在IL-HF-LPME方法中，将比例为3:1的[OMIM]PF6和月桂酸混合物固定在用作萃取溶剂的聚丙烯中空纤维孔中。研究并优化了一系列影响萃取效率的关键参数。在最佳条件下，3分钟内即可达到萃取平衡，线性良好，相关系数>0.9990，检测限在0.14至0.71 ng/mL之间，定量限在0.4至1.8 ng/mL之间，相对标准偏差在1.24 - 3.27%范围内（n = 5）。该方法应用于牛奶样品中四种PAHs的分析，回收率在93.6%至102.8%之间。
