基于DNA疫苗的多表位设计（初步报告）
Designing a DNA Vaccine-based Polytope (Preliminary Report).
作者信息
Jeibouei Shabnam, Bandehpour Mojgan, Kazemi Bahram, Haghighi Ali
机构信息
Dept. of Parasitology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
出版信息
Iran J Parasitol. 2017 Jul-Sep;12(3):441-445.
BACKGROUND
Leishmaniasis is a neglected disease affecting millions of people worldwide. The treatment of the disease is hampered due to high cost, toxicity and the crisis of drug resistance. Polytope approaches of genetic immunization could be a strategy for prevention of infectious diseases. Furthermore, the identification of genome sequence and the application of bioinformatics assist us to devise an effective vaccine's candidate.
METHODS
A linear sequence from predicted epitopes of GP63, LACK and CPC antigens was designed and was optimized using online available algorithms. The synthesized sequence (LAKJB93) was ligated to pEGFP-N1 plasmid.
RESULTS
The 264bp sequence was cloned at N terminal of GFP into pEGFP_N1 expression vector and transfect into CHO cell line. Construct was efficient expressed in CHO cells.
CONCLUSION
The protein of LAKJB93 cosnstruct was expressed in successfully.
背景
利什曼病是一种被忽视的疾病，影响着全球数百万人。由于成本高、毒性大以及耐药性危机，该疾病的治疗受到阻碍。基因免疫的多表位方法可能是预防传染病的一种策略。此外，基因组序列的鉴定和生物信息学的应用有助于我们设计出有效的疫苗候选物。
方法
设计了来自GP63、LACK和CPC抗原预测表位的线性序列，并使用在线可用算法进行了优化。将合成序列（LAKJB93）连接到pEGFP-N1质粒上。
结果
264bp序列在GFP的N末端克隆到pEGFP_N1表达载体中，并转染到CHO细胞系中。构建体在CHO细胞中高效表达。
结论
LAKJB93构建体的蛋白成功表达。
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