通过靶向LRIG2对miR-149-5p在黑色素瘤细胞存活和凋亡中的负调控作用
The negative regulation of miR-149-5p in melanoma cell survival and apoptosis by targeting LRIG2.
作者信息
Chen Wenqi, Zhang Jinhai, Xu Huijuan, Dai Jie, Zhang Xiaorong
机构信息
Department of Dermatology, Nanjing First Hospital, Nanjing Medical UniversityNanjing, Jiangsu, China.
Department of Epidemiology, Research Institute for Medicine of Nanjing CommandNanjing, Jiangsu, China.
出版信息
Am J Transl Res. 2017 Sep 15;9(9):4331-4340. eCollection 2017.
Abstract
MicroRNAs (miRNAs) are key regulators of diverse biological processes in tumor progression including melanoma. LRIG2 is reported as an oncogene in cancer, however, little is known regarding the molecular and functions of LRIG2 in melanoma. In this study, we reported that LRIG2 expression was higher in melanoma tissues and cell lines and was regulated by miR-149-5p. Furthermore, a luciferase reporter assay and rescue experiment indicated that miR-149-5p directly targeted LRIG2 by binding its 3'UTR. The overexpression of miR-149-5p significantly suppressed melanoma cell proliferation, colony formation, and promoted cell apoptosis. These results suggest that miR-149-5p acts as a suppressing molecule and may be a good method for melanoma therapy.
摘要
微小RNA（miRNA）是肿瘤进展（包括黑色素瘤）中多种生物学过程的关键调节因子。LRIG2在癌症中被报道为一种癌基因，然而，关于LRIG2在黑色素瘤中的分子机制和功能知之甚少。在本研究中，我们报道LRIG2在黑色素瘤组织和细胞系中表达较高，且受miR-149-5p调控。此外，荧光素酶报告基因实验和拯救实验表明，miR-149-5p通过结合LRIG2的3'非翻译区直接靶向LRIG2。miR-149-5p的过表达显著抑制黑色素瘤细胞增殖、集落形成，并促进细胞凋亡。这些结果表明，miR-149-5p作为一种抑制分子，可能是黑色素瘤治疗的一种有效方法。
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