Pierce C W, Sorensen C M, Kapp J A
Department of Pathology, Jewish Hospital, St. Louis, MO 63110.
J Immunol. 1988 Jul 1;141(1):64-70.
T cell subsets that regulate antibody responses to L-glutamic acid60-L-alanine30-L-tyrosine10 (GAT) in mice that are Ir gene non-responders have been further characterized. We previously defined several T cell subsets in GAT-primed non-responder mice. The Lyt-2+ suppressor-effector T cells suppress responses to GAT and GAT complexed to methylated BSA (GAT-MBSA). The Lyt-1+ cell population is complex and can be separated into I-J- Th cells, which support responses to GAT and GAT-MBSA. After priming, the Lyt-1+, I-J+ cell population contains suppressor-inducer cells that activate precursors of suppressor-effector cells to suppress responses to GAT and GAT-MBSA as well as Ts cells that directly inhibit responses to GAT but not GAT-MBSA. By contrast, the Lyt-1+ cells from virgin mice contain only cells that directly suppress responses to GAT but not GAT-MBSA. The major question addressed in the present studies was whether the Lyt-1+, I-J+ Ts cells in virgin and primed mice and the suppressor-inducer cells in GAT-primed mice were functionally and serologically distinct subsets. The studies used mAb and panning procedures to separate cell populations and inhibition of PFC cell responses to functionally define the activity of the cell populations. We used the following two mAb that were raised by immunizing rats with GAT-specific suppressor factors: 1248A4.10 (known to react with suppressor-inducer cells) and 1248A4.3, another reagent from the same fusion. Lyt-1+ cells from virgin spleens contained Ts cells that were A4.10-, A4.3+ and no suppressor-inducer T cells, whereas Lyt-1+ cells from GAT-primed spleens contained Ts cells that were A4.10-, A4.3+ as well as A4.10+, A4.3- suppressor-inducer cells. Thus, the Lyt1+, I-J+ cell subset can be divided into two functionally and serologically distinct subsets, direct Ts cells (1248A4.3+), which suppress responses to GAT but not GAT-MBSA, and GAT-primed suppressor-inducer T cells (1248A4.10+).
对Ir基因无反应小鼠中调节针对L-谷氨酸60-L-丙氨酸30-L-酪氨酸10（GAT）抗体反应的T细胞亚群进行了进一步的特性分析。我们之前在经GAT致敏的无反应小鼠中定义了几个T细胞亚群。Lyt-2⁺抑制效应T细胞抑制对GAT以及与甲基化牛血清白蛋白复合的GAT（GAT-MBSA）的反应。Lyt-1⁺细胞群体较为复杂，可分为I-J⁻辅助性T细胞，其支持对GAT和GAT-MBSA的反应。致敏后，Lyt-1⁺、I-J⁺细胞群体包含抑制诱导细胞，其激活抑制效应细胞的前体以抑制对GAT和GAT-MBSA的反应，以及直接抑制对GAT但不抑制对GAT-MBSA反应的Ts细胞。相比之下，来自未致敏小鼠的Lyt-1⁺细胞仅包含直接抑制对GAT反应但不抑制对GAT-MBSA反应的细胞。本研究中探讨的主要问题是未致敏和致敏小鼠中的Lyt-1⁺、I-J⁺ Ts细胞以及经GAT致敏小鼠中的抑制诱导细胞在功能和血清学上是否为不同的亚群。研究使用单克隆抗体和淘选程序分离细胞群体，并通过抑制PFC细胞反应来从功能上定义细胞群体的活性。我们使用了以下两种通过用GAT特异性抑制因子免疫大鼠产生的单克隆抗体：1248A4.10（已知与抑制诱导细胞反应）和1248A4.3，来自同一融合的另一种试剂。来自未致敏脾脏的Lyt-1⁺细胞包含A4.10⁻、A4.3⁺的Ts细胞且无抑制诱导T细胞，而来自经GAT致敏脾脏的Lyt-1⁺细胞包含A4.10⁻、A4.3⁺的Ts细胞以及A4.10⁺、A4.3⁻的抑制诱导细胞。因此，Lyt1⁺、I-J⁺细胞亚群可分为两个在功能和血清学上不同的亚群，即直接Ts细胞（1248A4.3⁺），其抑制对GAT但不抑制对GAT-MBSA的反应，以及经GAT致敏的抑制诱导T细胞（1248A4.10⁺）。
