Wang Xia, Astrof Sophie
Department of Medicine, Center for Translational Medicine, Sidney Kimmel Medical College of Thomas Jefferson University, Philadelphia, PA, USA.
Bio Protoc. 2017 Sep 5;7(17). doi: 10.21769/BioProtoc.2530.
Cardiac neural crest cells (CNCCs) originate at the dorsal edge of the neural tube between the otic pit and the caudal edge of the 3 somite, and migrate into the pharyngeal arches and the heart. We have shown that fibronectin (Fn1) plays an important role in the development of the CNCC by regulating the differentiation of CNCCs into vascular smooth muscle cells around pharyngeal arch arteries (Wang and Astrof, 2016). This protocol describes the isolation of CNCCs from the neural tube and from the caudal pharyngeal arches, and the differentiation of neural crest-derived cells into smooth muscle cells. This protocol was adapted from (Newgreen and Murphy, 2000; Pfaltzgraff , 2012).
心脏神经嵴细胞（CNCCs）起源于神经管的背侧边缘，位于耳窝和第三体节尾缘之间，并迁移至咽弓和心脏。我们已经表明，纤连蛋白（Fn1）通过调节CNCCs分化为咽弓动脉周围的血管平滑肌细胞，在CNCCs的发育中发挥重要作用（Wang和Astrof，2016）。本方案描述了从神经管和尾侧咽弓中分离CNCCs，以及将神经嵴衍生细胞分化为平滑肌细胞的方法。本方案改编自（Newgreen和Murphy，2000；Pfaltzgraff，2012）。
