Baba A, Nishiuchi Y, Uemura A, Tatsuno T, Iwata H
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University, Japan.
J Neurochem. 1988 Jul;51(1):237-42. doi: 10.1111/j.1471-4159.1988.tb04861.x.
The effect of forskolin on the excitatory amino acid-induced accumulation of cyclic AMP was examined in hippocampal preparations of the guinea pig. Forskolin at concentrations of 0.1-10 microM remarkably enhanced the stimulatory effects of histamine and adenosine, whereas it markedly attenuated the stimulation induced by cysteine sulfinate, an excitatory amino acid. Forskolin reduced the maximal response to cysteine sulfinate without affecting the apparent ED50. At a concentration of 1 microM, forskolin also inhibited the stimulatory effects of glutamate, N-methyl-DL-aspartate, and veratridine without affecting those of kainate and quisqualate. Pretreatment of the slices with 0.1 mM N-ethylmaleimide partially prevented the attenuation by forskolin of cysteine sulfinate-induced accumulation of cyclic AMP without affecting the stimulation induced by cysteine sulfinate. Forskolin at concentrations of less than 1 microM did not affect GTP-stimulated activity and Cl- -dependent activity of adenylate cyclase of the hippocampal membranes.
在豚鼠海马制备物中检测了福斯高林对兴奋性氨基酸诱导的环磷酸腺苷（cAMP）积累的影响。浓度为0.1 - 10微摩尔的福斯高林显著增强了组胺和腺苷的刺激作用，而它显著减弱了由兴奋性氨基酸半胱氨酸亚磺酸盐诱导的刺激。福斯高林降低了对半胱氨酸亚磺酸盐的最大反应，而不影响表观半数有效剂量（ED50）。在1微摩尔的浓度下，福斯高林还抑制了谷氨酸、N - 甲基 - DL - 天冬氨酸和藜芦碱的刺激作用，而不影响海人藻酸和喹啉酸的刺激作用。用0.1毫摩尔N - 乙基马来酰亚胺预处理切片部分阻止了福斯高林对半胱氨酸亚磺酸盐诱导的cAMP积累的减弱作用，而不影响半胱氨酸亚磺酸盐诱导的刺激。浓度低于1微摩尔的福斯高林不影响海马膜腺苷酸环化酶的GTP刺激活性和氯离子依赖性活性。
