Aix Marseille Univ, INSERM, VRCM, UMR_1076, UFR de Pharmacie, Marseille, France.
APHM, CHU La Conception, Department of Neonatology, Marseille, France.
Stem Cells Transl Med. 2017 Nov;6(11):1987-1996. doi: 10.1002/sctm.17-0074. Epub 2017 Oct 5.
Neonatal hypoxic-ischemic encephalopathy (NHIE) is a dramatic perinatal complication, associated with poor neurological prognosis despite neuroprotection by therapeutic hypothermia, in the absence of an available curative therapy. We evaluated and compared ready-to-use human umbilical cord blood cells (HUCBC) and bankable but allogeneic endothelial progenitors (ECFC) as cell therapy candidate for NHIE. We compared benefits of HUCBC and ECFC transplantation 48 hours after injury in male rat NHIE model, based on the Rice-Vannucci approach. Based on behavioral tests, immune-histological assessment and metabolic imaging of brain perfusion using single photon emission computed tomography (SPECT), HUCBC, or ECFC administration provided equally early and sustained functional benefits, up to 8 weeks after injury. These results were associated with total normalization of injured hemisphere cerebral blood flow assessed by SPECT/CT imaging. In conclusion, even if ECFC represent an efficient candidate, HUCBC autologous criteria and easier availability make them the ideal candidate for hypoxic-ischemic cell therapy. Stem Cells Translational Medicine 2017;6:1987-1996.
新生儿缺氧缺血性脑病（NHIE）是一种严重的围产期并发症，尽管通过治疗性低温进行神经保护，但在缺乏有效治疗方法的情况下，神经预后仍然较差。我们评估并比较了即用型人脐带血细胞（HUCBC）和可储存的同种异体内皮祖细胞（ECFC）作为 NHIE 的细胞治疗候选物。我们根据 Rice-Vannucci 方法，比较了损伤后 48 小时雄性大鼠 NHIE 模型中 HUCBC 和 ECFC 移植的益处。基于行为测试、免疫组织学评估以及使用单光子发射计算机断层扫描（SPECT）进行脑灌注代谢成像，HUCBC 或 ECFC 给药提供了同样的早期和持续的功能益处，直至损伤后 8 周。这些结果与 SPECT/CT 成像评估的损伤半球脑血流的完全正常化相关。总之，尽管 ECFC 是一种有效的候选物，但 HUCBC 具有自体标准和更易于获得的特点，使其成为缺氧缺血性细胞治疗的理想候选物。《干细胞转化医学》2017 年；6:1987-1996.
