Furtado L M, Da Silveira I C, Carneiro A C D M, Zehuri M M O N, Carboni S S C M, Tavares-Murta B M, Crema V O
Structural Biology Department, Institute of Natural and Biological Sciences, Federal University of TriânguloMineiro, Uberaba, MG, Brazil.
Pharmacology Department, Institute of Natural and Biological Sciences, Federal University of TriânguloMineiro, Uberaba, MG, Brazil.
Cell Mol Biol (Noisy-le-grand). 2017 Sep 30;63(9):13-17. doi: 10.14715/cmb/2017.63.9.3.
Cetuximab is a chimeric monoclonal antibody that acts as a competitive antagonist, by binding to EGFR. This cell signalling pathways regulates tumor progression. The oral squamous cell carcinoma undergoes to regional spreading and distant metastasis. This study aimed to evaluate the effect of treatment with Cetuximab on cell migration and invasion in OSCC cells, by using the SCC-4 cell line. Cell migration and cell invasion assay were performed and actin cytoskeleton of control and treated with Cetuximab cells were evaluated. Differences were considered significant when p<0.05.Cetuximab inhibited the migration of SCC-4 cells at three concentrations: 1 µg/mL, 50 µg/mL and 100 µg/mL (p<0.0001) in a dose-dependent manner. The number of SCC-4 treated cells with 1 μg/mL that migrated through the membrane was statistically different from 50 μg/mL (p<0.001) and 100 μg/mL (p<0.0001), and between 50 μg/mL and 100 μg/mL (p<0.01). Cetuximab 50 μg/mL inhibited cell invasion through the MatrigelTM compared with SCC-4 control cells (p<0.01). Cetuximab 50 μg/mL affected the organization of the actin cytoskeleton. Cetuximab has an inhibitory effect on actin cytoskeleton organization, cell migration and invasion, suggesting that Cetuximab treatment can be important to avoid oral squamous cell carcinoma metastasis.
西妥昔单抗是一种嵌合单克隆抗体，通过与表皮生长因子受体（EGFR）结合发挥竞争性拮抗剂的作用。这种细胞信号通路调节肿瘤进展。口腔鳞状细胞癌会发生局部扩散和远处转移。本研究旨在通过使用SCC - 4细胞系评估西妥昔单抗治疗对口腔鳞状细胞癌细胞迁移和侵袭的影响。进行了细胞迁移和细胞侵袭试验，并评估了对照组和经西妥昔单抗处理的细胞的肌动蛋白细胞骨架。当p<0.05时，差异被认为具有统计学意义。西妥昔单抗在1μg/mL、50μg/mL和100μg/mL这三种浓度下均以剂量依赖方式抑制SCC - 4细胞的迁移（p<0.0001）。用1μg/mL处理的SCC - 4细胞穿过膜迁移的数量与50μg/mL（p<0.001）和100μg/mL（p<0.0001）相比有统计学差异，且在50μg/mL和100μg/mL之间也有统计学差异（p<0.01）。与SCC - 4对照细胞相比，50μg/mL的西妥昔单抗抑制细胞通过基质胶侵袭（p<0.01）。50μg/mL的西妥昔单抗影响肌动蛋白细胞骨架的组织。西妥昔单抗对肌动蛋白细胞骨架组织、细胞迁移和侵袭具有抑制作用，这表明西妥昔单抗治疗对于避免口腔鳞状细胞癌转移可能具有重要意义。
