Gundogdu A, Kilic H, Kurekci C, Alp E
Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Erciyes University, Kayseri, Turkey.
Division of Metagenomic, Genome and Stem Cell Centre (GenKok), Erciyes University, Kayseri, Turkey.
Cell Mol Biol (Noisy-le-grand). 2017 Sep 30;63(9):80-86. doi: 10.14715/cmb/2017.63.9.15.
Quantitative data about extended-spectrum beta-lactamase producing E. coli (ESBLEC) in the wastewaters are scarce, especially in developing countries. These data could be useful to raise awareness about the potential risk of spreading ESBLEC strains in the community. Water samples were collected weekly over a 10-week period, from one urban sewage treatment plant (STP), one rural STP and one hospital complex's wastewater (HWW) in Turkey. Mean E. coli and ESBLEC loads were determined for each sampling point. For the 580 ESBLEC isolated, antimicrobial resistance profiles, phylogenetic grouping, presence of common beta-lactamese-typesand integrons were studied using PCR. The mean ESBLEC ratio was accounted for 0.58%, 0,12%, 1.53% of the total E. coli in urban, rural untreated wastewater and HWW, respectively. These values were higher for the outlets. The mean number of different antimicrobial classes to which the strains were resistant was highest in urban STP (4.0± 1.6). The antimicrobial resistance ratios of ESBLEC strains isolated from HWW were observed to be in between those of urban and rural STPs. The most common phylogenetic group was C composing (29.7%) and the most susceptible strains belonged to phylogroup B1. Wastewater treatments without sufficient decontamination, resulting in artificial selection of ESBLEC might lead to public health risk as these strains reach communities through environment. To avoid such risks and protect the human health as well as the environment, well-established decontamination measures imposing barriers against this artificial selection should be implemented.
关于废水中产超广谱β-内酰胺酶大肠杆菌（ESBLEC）的定量数据稀缺，尤其是在发展中国家。这些数据对于提高人们对社区中ESBLEC菌株传播潜在风险的认识可能有用。在土耳其，在10周的时间内每周从一个城市污水处理厂（STP）、一个农村污水处理厂和一个医院综合设施的废水（HWW）中采集水样。测定每个采样点的大肠杆菌和ESBLEC平均负荷。对于分离出的580株ESBLEC，使用PCR研究其抗菌药物耐药谱、系统发育分组、常见β-内酰胺酶类型和整合子的存在情况。城市、农村未经处理的废水和HWW中ESBLEC的平均比例分别占总大肠杆菌的0.58%、0.12%、1.53%。这些值在排放口处更高。城市污水处理厂中菌株对不同抗菌药物类别的平均耐药数量最高（4.0±1.6）。从医院综合设施废水中分离出的ESBLEC菌株的抗菌药物耐药率介于城市和农村污水处理厂之间。最常见的系统发育组是C组（占29.7%），最敏感的菌株属于B1系统发育组。如果污水处理没有足够的去污措施，导致对ESBLEC进行人工选择，那么当这些菌株通过环境传播到社区时可能会带来公共卫生风险。为避免此类风险并保护人类健康和环境，应实施完善的去污措施，对这种人工选择设置障碍。
