上皮-间质转化调控转录因子在头颈部鳞状细胞癌中的表达
Expression of Epithelial-Mesenchymal Transition Regulating Transcription Factors in Head and Neck Squamous Cell Carcinomas.
作者信息
Göppel Juliane, Möckelmann Nikolaus, Münscher Adrian, Sauter Guido, Schumacher Udo
机构信息
Department of Anatomy and Experimental Morphology, University Medical Center Hamburg-Eppendorf, Hamburg, Germany
Department of Ear, Nose and Throat Surgery, University Medical Center Hamburg-Eppendorf, Hamburg, Germany.
出版信息
Anticancer Res. 2017 Oct;37(10):5435-5440. doi: 10.21873/anticanres.11971.
BACKGROUND/AIM: The transcription factors Twist, Snail, Slug, ZEB1 and ZEB2 regulate epithelial-mesenchymal transition (EMT) and their expression has been associated with a poor prognosis in several cancer entities. The aim of this analysis was to investigate in parallel the expression of all of these transcription factors in head and neck squamous cell carcinomas (HNSCCs) in order to gain insight into their possible co-expression.
MATERIALS AND METHODS
Tumor tissue samples were immunohistochemically stained using antibodies against these transcription factors. The staining intensity and cellular distribution of the immunoreactivity was recorded.
RESULTS
In general, transcription factor immunoreactivity was noted in the nucleus of both cancer and stromal cells. The highest immunoreactivity was observed for Twist. Snail, Slug, ZEB1 and ZEB2 showed a much lesser immunoreactivity in cancer cells and they were expressed independently from each other.
CONCLUSION
Twist is the major transcription factor active in HNSCC; the other transcription factors of EMT seem to be of less importance in this tumor entity.
背景/目的：转录因子Twist、Snail、Slug、ZEB1和ZEB2调节上皮-间质转化（EMT），其表达与多种癌症实体的不良预后相关。本分析的目的是同时研究这些转录因子在头颈部鳞状细胞癌（HNSCC）中的表达情况，以便深入了解它们可能的共表达情况。
材料与方法
使用针对这些转录因子的抗体对肿瘤组织样本进行免疫组织化学染色。记录免疫反应的染色强度和细胞分布。
结果
总体而言，在癌细胞和基质细胞的细胞核中均观察到转录因子免疫反应性。Twist的免疫反应性最高。Snail、Slug、ZEB1和ZEB2在癌细胞中的免疫反应性要低得多，且它们彼此独立表达。
结论
Twist是在HNSCC中起作用的主要转录因子；EMT的其他转录因子在该肿瘤实体中似乎不太重要。
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