猪内分泌细胞中胰抑制素免疫反应性的定位

Localization of pancreastatin immunoreactivity in porcine endocrine cells.

作者信息

Ravazzola M, Efendic S, Ostenson C G, Tatemoto K, Hutton J C, Orci L

机构信息

Institute of Histology and Embryology, University of Geneva Medical School, Switzerland.

出版信息

Endocrinology. 1988 Jul;123(1):227-9. doi: 10.1210/endo-123-1-227.

DOI:10.1210/endo-123-1-227

PMID:2898359

Abstract

Pancreastatin is a peptide isolated from the porcine pancreas and shown to inhibit insulin release. We have studied the immunocytochemical distribution of pancreastatin in three porcine endocrine tissues: pancreas, gut, and adenohypophysis. Pancreastatin-specific immunoreactivity was found in all three locations and distributed to numerous cells. In the pancreas, we performed the alternate labeling of consecutive thick (immunofluorescence) or thin (protein A-gold) sections and we observed that pancreastatin colocalizes to secretory granules of insulin and somatostatin-containing cells. The relationship of pancreastatin to chromogranin A is discussed.

摘要

胰抑制素是一种从猪胰腺中分离出来的肽，已证实其能抑制胰岛素释放。我们研究了胰抑制素在猪的三种内分泌组织（胰腺、肠道和腺垂体）中的免疫细胞化学分布。在所有这三个部位均发现了胰抑制素特异性免疫反应性，且分布于众多细胞中。在胰腺中，我们对连续的厚切片（免疫荧光）或薄切片（蛋白A-金标法）进行交替标记，观察到胰抑制素与胰岛素及含生长抑素细胞的分泌颗粒共定位。文中还讨论了胰抑制素与嗜铬粒蛋白A的关系。