Srivastava U S, Goswami T
Département de Nutrition, Université de Montréal, Québec, Canada.
Exp Biol. 1988;47(3):185-93.
The proximate composition of skeletal muscle and liver and the incorporation of radioactive glutamate into their various components were investigated in vitamin E-deficient rabbits and genetically dystrophic (dy/dy) mice during various stages of the disease. Total fat content in skeletal muscle was decreased in the early phase and increased in the terminal stage of both types of dystrophy. This component altered only in the liver of dystrophic mice, showing a reduction in the mild stage but returning later to normal values. Carbohydrate content was diminished in the skeletal muscle of both species at each stage of the disease. In the liver, however, it was elevated considerably in vitamin E-deficient rabbits, but was depressed in dystrophic mice. Protein content was decreased in the skeletal muscle of both dystrophic models. Similar results were obtained for amino acids, except that they were increased in the terminal stages of nutritional dystrophy. Analogous values were recorded for hepatic amino acids in nutritionally-induced and hereditary dystrophy (in rabbits and mice respectively). Incorporation of C14-glutamate into various components of skeletal muscle and liver in both models revealed divergent responses, depending upon the nature of the dystrophy, and the component in which incorporation was measured. These data indicate a rapid turnover of C14-glutamate in the skeletal muscle and liver of vitamin E-deficient and genetically dystrophic animals. Also, it is clear that the changes in the various components of skeletal muscle and liver, as well as the pattern of C14-glutamate incorporation, are different in the two types of dystrophy.
在维生素E缺乏的兔子和遗传性肌营养不良（dy/dy）小鼠疾病的不同阶段，研究了骨骼肌和肝脏的近似组成以及放射性谷氨酸掺入它们各种成分的情况。在两种类型的肌营养不良症的早期，骨骼肌中的总脂肪含量降低，而在末期则增加。这一成分仅在肌营养不良小鼠的肝脏中发生变化，在轻度阶段减少，但随后恢复到正常值。在疾病的每个阶段，两种动物骨骼肌中的碳水化合物含量均减少。然而，在肝脏中，维生素E缺乏的兔子的碳水化合物含量显著升高，而肌营养不良小鼠的则降低。两种肌营养不良模型的骨骼肌中的蛋白质含量均降低。氨基酸也得到了类似的结果，只是在营养性肌营养不良的末期有所增加。在营养性诱导和遗传性肌营养不良（分别在兔子和小鼠中）中，肝脏氨基酸也记录到了类似的值。在两种模型中，将C14-谷氨酸掺入骨骼肌和肝脏的各种成分中显示出不同的反应，这取决于肌营养不良的性质以及测量掺入的成分。这些数据表明，在维生素E缺乏和遗传性肌营养不良的动物的骨骼肌和肝脏中，C14-谷氨酸的周转很快。此外，很明显，在两种类型的肌营养不良中，骨骼肌和肝脏各种成分的变化以及C14-谷氨酸掺入的模式是不同的。
