Squires E L, Pickett B W, Amann R P
J Reprod Fertil Suppl. 1979(27):7-12.
Five ejaculates were collected at hourly intervals from 32 sexually rested stallions. Gel volume, total seminal volume, sperm concentration and spermatozoa per ejaculate declined (P less than 0.01) from the first to the second or third ejaculate. Gel-free seminal volume or percentage of motile spermatozoa did not vary (P less than 0.05) among ejaculates. Ejaculates from 2- to 3-year-old stallions contained less volume and fewer spermatozoa than those from 9- to 16-year-old stallions. Regardless of the stallion's age the first, first 2, first 3 and first 4 ejaculates represented 50, 74, 86 and 93% of the total numbers of spermatozoa contained in the 5 ejaculates. In addition, the number of spermatozoa that might be obtained in 5 ejaculates can be predicted by multiplying the total number of spermatozoa in two successive ejaculates by 1.35 +/- 0.03. Although some fifth ejaculates contained as few as 0.02 X 10(9) motile spermatozoa, the mean number of motile spermatozoa in fifth ejaculates from sexually rested stallions was 0.52 X 10(9) spermatozoa; presumably enough to impregnate a mare during natural service.
从32匹性休息的种马中每隔一小时采集一次射精样本，共采集5次。从第一次射精到第二次或第三次射精，凝胶体积、精液总体积、精子浓度和每次射精的精子数量均下降（P<0.01）。无凝胶精液体积或活动精子百分比在各次射精之间无差异（P<0.05）。2至3岁种马的射精量和精子数量比9至16岁种马的少。无论种马年龄如何，第一次、前两次、前三次和前四次射精分别占5次射精中精子总数的50%、74%、86%和93%。此外，5次射精中可能获得的精子数量可以通过将连续两次射精中的精子总数乘以1.35±0.03来预测。虽然有些第五次射精中的活动精子数量低至0.02×10⁹，但性休息种马的第五次射精中活动精子的平均数量为0.52×10⁹个精子；据推测，这足以在自然交配期间使母马受孕。
