Abdul Momin Muhd Haziq F, Bean David C, Hendriksen Rene S, Haenni Marisa, Phee Lynette M, Wareham David W
Antimicrobial Research Group, Blizard Institute, Barts and The London School of Medicine and Dentistry, Queen Mary University of London, London, UK.
School of Applied and Biomedical Sciences, Federation University Australia, Ballarat, Australia.
J Med Microbiol. 2017 Nov;66(11):1554-1561. doi: 10.1099/jmm.0.000602. Epub 2017 Oct 6.
A selective chromogenic culture medium for the laboratory isolation and differentiation of colistin resistant Acinetobacter, Pseudomonas, Stenotrophomonas and Enterobacteriaceae spp. (CHROMagar COL-APSE) was developed, evaluated and compared to an existing selective bacterial culture medium (SuperPolymyxin).
The medium was challenged with 84 isolates, including polymyxin B (POL B)-susceptible and -resistant type strains and colistin (COL)-resistant organisms recovered from human and animal samples. Susceptibility to COL and POL B was determined by agar dilution and broth microtitre dilution. The lower limit for the detection of COL-resistant organisms was also calculated for both CHROMagar COL-APSE and SuperPolymyxin media. The ability to isolate and correctly differentiate COL-resistant organisms within mixed cultures was also assessed and compared using both media.
Using CHROMagar COL-APSE, Gram-negative pathogens (n=71) with intrinsic (n=8) or acquired COL (n=63) resistance were recovered with 100 % specificity down to the lower limit of detection of 10 colony-forming units (c.f.u.). The growth on SuperPolymyxin was similar, but notably weaker for COL-resistant non-fermentative bacteria (Acinetobacter, Pseudomonas and Stenotrophomonas). CHROMagar COL-APSE was also more sensitive in supporting the growth of Enterobacteriaceae with COL resistance associated with the carriage of mcr-1.
CHROMagar COL-APSE is a sensitive and specific medium for the growth of COL-resistant bacterial pathogens. Due to the low limit of detection (10 c.f.u.), it may be useful as a primary isolation medium in the surveillance and recovery of COL-resistant bacteria from complex human, veterinary and environmental samples, especially those with plasmid-mediated MCR-1 or novel mechanisms of polymyxin resistance.
开发了一种用于实验室分离和鉴别耐黏菌素不动杆菌、假单胞菌、嗜麦芽窄食单胞菌和肠杆菌科细菌的选择性显色培养基（CHROMagar COL-APSE），并对其进行评估，同时与现有的选择性细菌培养基（SuperPolymyxin）进行比较。
用84株分离菌对该培养基进行测试，包括多黏菌素B（POL B）敏感和耐药的标准菌株以及从人和动物样本中分离出的耐黏菌素（COL）的菌株。通过琼脂稀释法和肉汤微量稀释法测定对COL和POL B的敏感性。还计算了CHROMagar COL-APSE和SuperPolymyxin培养基检测耐COL菌株的下限。同时评估并比较了两种培养基在混合培养物中分离和正确鉴别耐COL菌株的能力。
使用CHROMagar COL-APSE，可100%特异性地分离出具有固有（n=8）或获得性COL耐药（n=63）的革兰氏阴性病原体（n=71），检测下限低至10个菌落形成单位（c.f.u.）。在SuperPolymyxin上的生长情况相似，但耐COL的非发酵菌（不动杆菌、假单胞菌和嗜麦芽窄食单胞菌）的生长明显较弱。CHROMagar COL-APSE在支持携带mcr-1的耐COL肠杆菌科细菌生长方面也更敏感。
CHROMagar COL-APSE是一种用于耐COL细菌病原体生长的敏感且特异的培养基。由于检测下限低（10 c.f.u.），它可作为从复杂的人、兽医和环境样本，特别是那些具有质粒介导的MCR-1或新的多黏菌素耐药机制的样本中监测和分离耐COL细菌的初代分离培养基。
