从太湖分离出的一种新型本土鞘氨醇单胞菌属微囊藻毒素降解菌。

A Novel and Native Microcystin-Degrading Bacterium of Sphingopyxis sp. Isolated from Lake Taihu.

作者信息

Zhang Juan, Lu Qingqing, Ding Qin, Yin Lihong, Pu Yuepu

机构信息

Key Laboratory of Environmental Medicine Engineering of Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.

出版信息

Int J Environ Res Public Health. 2017 Oct 6;14(10):1187. doi: 10.3390/ijerph14101187.

A native, highly efficient microcystin-LR (MC-LR)-degrading bacterium named a7 was isolated from Lake Taihu and identified as Sphingopyxis sp. by 16S rDNA sequence analysis. The strain a7 could totally degrade MC-LR at a rate of 3.33 mg/(L•h), as detected by high-performance liquid chromatography (HPLC). The mlrA, mlrC, and mlrD genes were detected in the strain a7 by sequence analysis. Tetrapeptide and Adda-which are the middle metabolites of MC-LR-were analyzed via liquid chromatography time-of-flight mass spectrometry (LC-TOF-MS) during degradation. These metabolites were degraded completely, which suggested that the native Sphingopyxis sp. a7 was highly efficient in MC-LR degradation under bench conditions. Thus, strain a7 exhibited a significant potential application for bioremediation in water bodies contaminated by MC-LR produced by harmful cyanobacterial blooms.

从太湖分离出一株天然高效降解微囊藻毒素-LR（MC-LR）的细菌，命名为a7，并通过16S rDNA序列分析鉴定为鞘氨醇单胞菌属（Sphingopyxis sp.）。通过高效液相色谱（HPLC）检测，菌株a7能够以3.33 mg/(L•h)的速率完全降解MC-LR。通过序列分析在菌株a7中检测到mlrA、mlrC和mlrD基因。在降解过程中，通过液相色谱飞行时间质谱（LC-TOF-MS）分析了作为MC-LR中间代谢产物的四肽和Adda。这些代谢产物被完全降解，这表明天然鞘氨醇单胞菌属a7在实验室条件下对MC-LR的降解效率很高。因此，菌株a7在受有害蓝藻水华产生的MC-LR污染的水体生物修复中具有显著的潜在应用价值。