Zhang Juan, Lu Qingqing, Ding Qin, Yin Lihong, Pu Yuepu
Key Laboratory of Environmental Medicine Engineering of Ministry of Education, School of Public Health, Southeast University, Nanjing 210009, China.
Int J Environ Res Public Health. 2017 Oct 6;14(10):1187. doi: 10.3390/ijerph14101187.
A native, highly efficient microcystin-LR (MC-LR)-degrading bacterium named a7 was isolated from Lake Taihu and identified as Sphingopyxis sp. by 16S rDNA sequence analysis. The strain a7 could totally degrade MC-LR at a rate of 3.33 mg/(L•h), as detected by high-performance liquid chromatography (HPLC). The mlrA, mlrC, and mlrD genes were detected in the strain a7 by sequence analysis. Tetrapeptide and Adda-which are the middle metabolites of MC-LR-were analyzed via liquid chromatography time-of-flight mass spectrometry (LC-TOF-MS) during degradation. These metabolites were degraded completely, which suggested that the native Sphingopyxis sp. a7 was highly efficient in MC-LR degradation under bench conditions. Thus, strain a7 exhibited a significant potential application for bioremediation in water bodies contaminated by MC-LR produced by harmful cyanobacterial blooms.
从太湖分离出一株天然高效降解微囊藻毒素-LR（MC-LR）的细菌，命名为a7，并通过16S rDNA序列分析鉴定为鞘氨醇单胞菌属（Sphingopyxis sp.）。通过高效液相色谱（HPLC）检测，菌株a7能够以3.33 mg/(L•h)的速率完全降解MC-LR。通过序列分析在菌株a7中检测到mlrA、mlrC和mlrD基因。在降解过程中，通过液相色谱飞行时间质谱（LC-TOF-MS）分析了作为MC-LR中间代谢产物的四肽和Adda。这些代谢产物被完全降解，这表明天然鞘氨醇单胞菌属a7在实验室条件下对MC-LR的降解效率很高。因此，菌株a7在受有害蓝藻水华产生的MC-LR污染的水体生物修复中具有显著的潜在应用价值。
