Centre of Molecular and Macromolecular Studies, Polish Academy of Sciences, Sienkiewicza 112, 90-363 Lodz, Poland.
Univ Paris Diderot, Sorbonne Paris Cité, ITODYS, UMR CNRS 7086, 15 rue JA De Baïf, 75205 Paris, France; Université Paris Est, UMR 7182 CNRS, UPEC, 94320 Thiais, France.
Colloids Surf B Biointerfaces. 2017 Dec 1;160:438-445. doi: 10.1016/j.colsurfb.2017.09.054. Epub 2017 Sep 25.
We investigated the distribution of polyglycidol and polystyrene on the surface of poly(styrene/α-tert-butoxy-ω-vinylbenzyl-polyglycidol) microspheres (random distribution or segregated into hydrophilic and hydrophobic patches), using fibrinogen (Fb) as a macromolecular probe. The fibrinogen was adsorbed or covalently attached to the surface of the poly(styrene-co-α-tert-butoxy-ω-vinylbenzyl-polyglycidol) (P(S/PGLy)) microspheres. The P(S/PGLy) particles were prepared by emulsion copolymerization of styrene and α-tert-butoxy-ω-vinylbenzyl-polyglycidol (PGLy) macromonomer initiated with potassium persulfate. The polymerizations yielded P(S/PGLy) particles with various surface fractions of polyglycidol, depending on the amount of added macromonomer and the addition process. In some syntheses, the entire macromonomer amount was added once at the beginning of the polymerization, while in others, the macromonomer was added gradually after the formation of particle seeds from pure polystyrene. XPS studies revealed that the fraction of polyglycidol in the interfacial layer of the microspheres was larger when the entire amount of macromonomer was added at the beginning of the polymerization than when it was added after formation of the polystyrene seeds. Studies of fibrinogen adsorption provided the first evidence of segregation of the hydrophobic (polystyrene) and hydrophilic (polyglycidol) components at the surface of the composite P(S/PGLy) microspheres into patches. The hydrophobic patches are composed mainly of polystyrene. However, they also contain a small amount of polyglycidol chains, making the adsorption of fibrinogen weaker than the adsorption onto the pure polystyrene. Studies of covalent immobilization of fibrinogen on the microspheres via 1,3,5-trichlorotriazine confirmed these findings.
我们研究了聚环氧甘油和聚苯乙烯在聚（苯乙烯/α-叔丁氧基-ω-乙烯基苄基-聚环氧甘油）微球表面的分布（随机分布或分离成亲水区和疏水区），使用纤维蛋白原（Fb）作为大分子探针。纤维蛋白原被吸附或共价连接到聚（苯乙烯-co-α-叔丁氧基-ω-乙烯基苄基-聚环氧甘油）（P（S/PGLy））微球的表面。P（S/PGLy）粒子通过苯乙烯和α-叔丁氧基-ω-乙烯基苄基-聚环氧甘油（PGLy）大分子单体的乳液共聚制备，引发剂为过硫酸钾。聚合得到的 P（S/PGLy）粒子具有不同的聚环氧甘油表面分数，这取决于添加的大分子单体的量和添加方式。在一些合成中，整个大分子单体的量在聚合开始时一次性添加，而在其他合成中，大分子单体在从纯聚苯乙烯形成粒子种子后逐渐添加。XPS 研究表明，当整个大分子单体的量在聚合开始时一次性添加时，微球界面层中聚环氧甘油的分数大于在形成聚苯乙烯种子后添加时的分数。纤维蛋白原吸附研究提供了第一个证据，表明疏水（聚苯乙烯）和亲水（聚环氧甘油）成分在复合 P（S/PGLy）微球表面分离成斑块。疏水斑块主要由聚苯乙烯组成。然而，它们也含有少量的聚环氧甘油链，使得纤维蛋白原的吸附比吸附到纯聚苯乙烯上弱。通过 1,3,5-三氯均三嗪对微球上纤维蛋白原的共价固定研究证实了这些发现。
