Chaiwongkot Arkom, Niruthisard Somchai, Kitkumthorn Nakarin, Bhattarakosol Parvapan
Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Department of Obstetrics and Gynecology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Diagn Microbiol Infect Dis. 2017 Dec;89(4):265-270. doi: 10.1016/j.diagmicrobio.2017.08.010. Epub 2017 Aug 25.
Human papillomavirus 16 is the most prevalent type found in cervical cancer worldwide, accounting for >50% of all cases. Quantitative methylation analysis of human papillomavirus 16L1 gene within 5' (CpGs 5600, 5606, 5609, 5615) and 3' (7136 and 7145) regions to determine potential biomarker for cervical cancer progression was performed in exfoliated cervical cells collected from 101 Thai women of precancerous and cancerous lesions. Intermediate to high methylation levels (>20%) were detected in HPV16 5'L1 regions especially CpG 5600 of all cancerous (100%) and 50% of CIN3 samples, whereas normal/CIN1 samples (80%) showed methylation levels <20%. Our results indicate the potential use of HPV 16L1 gene methylation at specific site as a biomarker for prognostic cervical cancer screening, however, suitable cutoff should be further evaluated in a larger sample size.
人乳头瘤病毒16型是全球宫颈癌中最常见的类型，占所有病例的50%以上。对101名患有癌前病变和癌性病变的泰国女性宫颈脱落细胞进行了人乳头瘤病毒16L1基因5'（CpGs 5600、5606、5609、5615）和3'（7136和7145）区域的定量甲基化分析，以确定宫颈癌进展的潜在生物标志物。在HPV16 5'L1区域检测到中等至高甲基化水平（>20%），尤其是在所有癌性样本（100%）和50%的CIN3样本中的CpG 5600，而正常/CIN1样本（80%）的甲基化水平<20%。我们的结果表明，HPV 16L1基因在特定位点的甲基化有可能作为宫颈癌预后筛查的生物标志物，然而，应在更大样本量中进一步评估合适的临界值。
