Li Jianchao, Liu Yifei, Xue Jianhua, Xu Mingming, Zhang Jianguo, Liu Junhua, Wang Wenyi
Department of Thoracic surgery, the First People's Hospital of Yancheng, 166 Yulongxi Road, Yancheng, Jiangsu, 224006, China.
Department of Thoracic surgery, the Affiliated Hospital of Nantong University, 20 Xisi Road, Nantong, Jiangsu, 226001, China.
Pathol Oncol Res. 2019 Jan;25(1):115-121. doi: 10.1007/s12253-017-0321-4. Epub 2017 Oct 6.
Krüppel-like factor 8 (KLF8) plays a key role in cancer progression. However, its expression pattern and relationship with clinicopathological characteristics in non-small cell lung cancer (NSCLC) has not been completely elucidated. The study aimed to investigate the expression of KLF8 and its correlation with clinical pathologic features in NSCLC and to explore the potential mechanism. The expression of KLF8 in NSCLC was detected by RT-PCR, Western blot and immunohistochemistry. The expression of Vimentin and E-cadherin, epithelial-mesenchymal transition (EMT) markers, were detected by immunohistochemistry in the NSCLC. The relationship between KLF8 expression and various clinicopathological features or EMT markers was investigated. The results showed m-RNA and protein of KLF8 were overexpressed in NSCLC and the percent of KLF8 positive cells was positively correlated with TNM stage, lymph node metastasis and poor overall survive. Moreover, high expression of KLF8 correlated with E-cadherin low expression, and Vimentin overexpression. Additionally, COX multivariate regression analysis suggested that TNM stage, KLF8, E-cadherin and Vimentin were independent prognostic indicators for overall survival of patients with NSCLC. The data demonstrate that KLF8 is overexpressed in NSCLC. KLF8 overexpression promotes the malignancy of NSCLC, which mechanism may be involved in EMT. KLF8 maybe serve as a potential therapeutic target for NSCLC.
Krüppel样因子8（KLF8）在癌症进展中起关键作用。然而，其在非小细胞肺癌（NSCLC）中的表达模式及其与临床病理特征的关系尚未完全阐明。本研究旨在探讨KLF8在NSCLC中的表达及其与临床病理特征的相关性，并探索其潜在机制。采用逆转录-聚合酶链反应（RT-PCR）、蛋白质免疫印迹法和免疫组织化学法检测NSCLC中KLF8的表达。采用免疫组织化学法检测NSCLC中波形蛋白和E-钙黏蛋白（上皮-间质转化（EMT）标志物）的表达。研究KLF8表达与各种临床病理特征或EMT标志物之间的关系。结果显示，KLF8的信使核糖核酸（m-RNA）和蛋白在NSCLC中过表达，KLF8阳性细胞百分比与TNM分期、淋巴结转移及总体生存率差呈正相关。此外，KLF8高表达与E-钙黏蛋白低表达及波形蛋白过表达相关。另外，COX多因素回归分析表明，TNM分期、KLF8、E-钙黏蛋白和波形蛋白是NSCLC患者总体生存的独立预后指标。数据表明，KLF8在NSCLC中过表达。KLF8过表达促进NSCLC的恶性程度，其机制可能与EMT有关。KLF8可能是NSCLC的一个潜在治疗靶点。
