蛋白质阵列II：抗原阵列

Protein Arrays II: Antigen Arrays.

作者信息

Yuan Yulin, Wang Hongting, Lin Zuan-Tao, Hong Xia, Heon Mikala, Wu Tianfu

机构信息

Department of Biomedical Engineering, University of Houston, 3605 Cullen Blvd, Houston, TX, 77204-5060, USA.

Department of Clinical Laboratory, The People's Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, China.

出版信息

Methods Mol Biol. 2017;1654:271-277. doi: 10.1007/978-1-4939-7231-9_20.

DOI:10.1007/978-1-4939-7231-9_20

PMID:28986798

Abstract

Antigen arrays are fabricated using various antigens such as DNA, histones, synthetic peptides, recombinant proteins, or cell extracts to detect autoantibodies in autoimmune diseases, alloantibodies in transplantation, drug-induced antibodies or cancer-induced antibodies in blood or cell culture supernatant. In this protocol, we will provide a step-by-step executable procedure to perform antigen arrays, including antigen preparation and printing, blocking, sample loading, array detection, imaging, and data analysis.

摘要

抗原阵列是利用各种抗原构建的，如DNA、组蛋白、合成肽、重组蛋白或细胞提取物，用于检测自身免疫性疾病中的自身抗体、移植中的同种抗体、血液或细胞培养上清液中的药物诱导抗体或癌症诱导抗体。在本方案中，我们将提供一个执行抗原阵列的逐步可操作程序，包括抗原制备与打印、封闭、样品加载、阵列检测、成像和数据分析。