星形胶质细胞中功能性N-甲基-D-天冬氨酸受体的分析

Analysis of Functional NMDA Receptors in Astrocytes.

作者信息

Kirchhoff Frank

机构信息

Molecular Physiology, Center for Integrative Physiology and Molecular Medicine (CIPMM), University of Saarland, Building 48, D-66421, Homburg, Germany.

出版信息

Methods Mol Biol. 2017;1677:241-251. doi: 10.1007/978-1-4939-7321-7_13.

DOI:10.1007/978-1-4939-7321-7_13

PMID:28986877

Abstract

Neuronal N-methyl-D-aspartate (NMDA) receptors are well known for their pivotal role in memory formation. Originally, they were thought to be exclusive to neurons. However, numerous studies revealed their functional expression also on various types of glial cells in the nervous system. Here, the methodology how to study the physiology of NMDA receptors selectively on astrocytes will be described in detail. Astrocytes are the main class of neuroglia that control transmitter and ion homeostasis, which link cerebral blood flow and neuronal energy demands, but also affect synaptic transmission directly.

摘要

神经元N-甲基-D-天冬氨酸（NMDA）受体因其在记忆形成中的关键作用而广为人知。最初，人们认为它们仅存在于神经元中。然而，大量研究表明它们在神经系统的各种类型神经胶质细胞上也有功能性表达。在此，将详细描述如何在星形胶质细胞上选择性研究NMDA受体生理学的方法。星形胶质细胞是神经胶质的主要类型，可控制递质和离子稳态，连接脑血流量与神经元能量需求，还能直接影响突触传递。