Wagner Vivian Petersen, Martins Manoela Domingues, Castilho Rogerio Moraes
Department of Oral Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, RS, 90035-003, Brazil.
Laboratory of Epithelial Biology, Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI, 48109-1078, USA.
Methods Mol Biol. 2018;1692:179-193. doi: 10.1007/978-1-4939-7401-6_16.
Chromatin decondensation is a key mechanism that guarantees gene transcription and repair of the genome, regulated mainly by the acetylation of histones. Emerging evidence has pointed out to histones as a new controlling mechanism of stem cell maintenance and fate. In this chapter, we will focus on the methods used to enrich tumor cell lines for cancer stem cells, and in the methods to identify the status of the histone acetylation in cancer cells and stem cells using immunofluorescence, invasion, and adhesion assays and identification of nuclear size.
染色质解聚是保证基因转录和基因组修复的关键机制，主要受组蛋白乙酰化调控。新出现的证据表明，组蛋白是干细胞维持和命运的一种新的控制机制。在本章中，我们将重点介绍用于富集肿瘤细胞系中的癌症干细胞的方法，以及使用免疫荧光、侵袭和黏附测定以及核大小鉴定来识别癌细胞和干细胞中组蛋白乙酰化状态的方法。
