Intartaglia Martina, Sabetta Rosalaura, Gargiulo Monica, Roncador Giovanna, Marino Federica Zito, Franco Renato
Pathology Unit, University of Campania "Luigi Vanvitelli", Via Luciano Armanni, 20, 80138, Naples, Italy.
Monoclonal Antibody Unit, Spanish National Cancer Research Center, Madrid, Spain.
Methods Mol Biol. 2018;1692:195-211. doi: 10.1007/978-1-4939-7401-6_17.
Cancer stem cells (CSCs) are rare immortal cells within a tumor that can self-renew and drive tumorigenesis. CSCs play a pivotal role in the tumor development, progression and relapse, as well as in the resistance of anticancer therapy. Different tools could help in the analysis of CSCs, especially Immunohistochemistry (IHC) represents a useful technique able to identify several specific CSC markers. The main aims of this chapter are the description of the explain immunohistochemical methods used in the characterization of CSCs. Furthermore, focus on the most common troubleshooting in CSCs IHC is provided, especially the pitfalls of the CSCs markers IHC on tissue microarrays.
癌症干细胞（CSCs）是肿瘤内罕见的永生细胞，能够自我更新并驱动肿瘤发生。癌症干细胞在肿瘤发展、进展和复发以及抗癌治疗耐药性方面发挥着关键作用。不同的工具有助于癌症干细胞的分析，尤其是免疫组织化学（IHC）是一种能够识别多种特定癌症干细胞标志物的有用技术。本章的主要目的是描述用于癌症干细胞表征的免疫组织化学方法。此外，还重点介绍了癌症干细胞免疫组织化学中最常见的故障排除方法，尤其是组织微阵列上癌症干细胞标志物免疫组织化学的陷阱。
